Evaluation of micronuclei, cytomorphometric and cytologic changes of the oral mucosa in hookah and cigarette smokers.

Autor: Sepulveda Inostroza EA; Technology Research Center (NPT), Universidade Mogi das Cruzes, Mogi das Cruzes, Brazil; School of Dentistry, Universidade Mogi das Cruzes, Mogi das Cruzes, Brazil., Bressane A; Department of Environmental Engineering, Institute of Science and Technology, São Paulo State University (Unesp), São José dos Campos, São Paulo, Brazil., Schwarzmeier LÂT; School of Dentistry, Universidade Mogi das Cruzes, Mogi das Cruzes, Brazil., Lacerda EB; School of Dentistry, Universidade Mogi das Cruzes, Mogi das Cruzes, Brazil., Anjos KRD; Research Center for Lasers and Applications - Nuclear and Energy Research Institute (IPEN)/University of São Paulo (USP), São Paulo, Brazil., Santos TSPD; School of Dentistry, Universidade Mogi das Cruzes, Mogi das Cruzes, Brazil., Cavalcanti DR; School of Dentistry, Universidade Mogi das Cruzes, Mogi das Cruzes, Brazil., Nascimento FD; Department of Biochemistry, Molecular Biology Division, Federal University of São Paulo (UNIFESP), São Paulo, Brazil., Almeida JD; Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (Unesp), São José dos Campos, São Paulo, Brazil., Oliveira Alves MG; Technology Research Center (NPT), Universidade Mogi das Cruzes, Mogi das Cruzes, Brazil; Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (Unesp), São José dos Campos, São Paulo, Brazil. Electronic address: mgoliveiraalves@gmail.com.
Jazyk: angličtina
Zdroj: Oral surgery, oral medicine, oral pathology and oral radiology [Oral Surg Oral Med Oral Pathol Oral Radiol] 2024 Jun; Vol. 137 (6), pp. 640-650. Date of Electronic Publication: 2024 Mar 28.
DOI: 10.1016/j.oooo.2024.03.009
Abstrakt: Objective: To analyze the effect of hookah and cigarettes on the oral mucosa of smokers through the use of exfoliative cytology.
Study Design: Smear samples were collected by exfoliative cytology from the tongue of 33 hookah smokers, 22 cigarette smokers, and 30 non-smokers. The selected analyses include micronuclei (MN), metanuclear anomalies, epithelial maturation, and cytomorphology (nuclear area [NA], cytoplasmic area [CA], and NA/CA ratio).
Results: The largest differences observed for MN and metanuclear anomalies were between cigarette smokers and the control group (notably 1 MN P = .04; total cells with MN P = .039; total MN P = .042; karyorrhexis and binucleation, P = .0001). The hookah group, compared with the control group, showed the greatest differences for karyolysis (P = .0023), binucleation (P = .0003), and broken egg (P = .008). Significant differences were found between the smokers and the control groups regarding changes in the superficial cell without nucleus, perinuclear halo, vacuolization, color change, mucus, and keratohyalin granules. There was a significant increase in the NA and NA/CA ratio in the smoker groups.
Conclusion: This study showed that a combined analysis of exfoliative cytology associated with other diagnostic methods is a useful tool for studying oral carcinogenesis. Hookah and cigarettes showed similar effects in terms of displaying substantial cytogenetic and cytotoxic damage.
Competing Interests: Disclosure None.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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