Magnetic carbon nanotubes modified with proteins and hydrophilic monomers: Cytocompatibility, in-vitro toxicity assays and permeation across biological interfaces.

Autor: Rosa MA; Laboratory of Toxicant and Drug Analyses, Faculty of Pharmaceutical Sciences, Federal University of Alfenas, 37130-001 Alfenas, MG, Brazil., Granja A; LAQV, REQUIMTE, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Rua Jorge de Viterbo Ferreira, 228, 4050-313 Porto, Portugal., Nunes C; LAQV, REQUIMTE, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Rua Jorge de Viterbo Ferreira, 228, 4050-313 Porto, Portugal., Reis S; LAQV, REQUIMTE, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Rua Jorge de Viterbo Ferreira, 228, 4050-313 Porto, Portugal., da Silva ABS; Spectrometry, Sample Preparation and Mechanization Group, Institute of Chemistry, University of Campinas - Unicamp, P.O. Box 6154, Campinas, SP 13083-970, Brazil., Leal KNDS; Spectrometry, Sample Preparation and Mechanization Group, Institute of Chemistry, University of Campinas - Unicamp, P.O. Box 6154, Campinas, SP 13083-970, Brazil., Arruda MAZ; Spectrometry, Sample Preparation and Mechanization Group, Institute of Chemistry, University of Campinas - Unicamp, P.O. Box 6154, Campinas, SP 13083-970, Brazil., Gorup LF; Institute of Chemistry, Federal University of Alfenas, 37130-001 Alfenas, MG, Brazil; School of Chemistry and Food Science, Federal University of Rio Grande, Av. Italia km 8 Bairro Carreiros, 96203-900 Rio Grande, RS, Brazil; Materials Engineering, Federal University of Pelotas, Campus Porto, 96010-610 Pelotas, RS, Brazil., Santos MG; Laboratory of Toxicant and Drug Analyses, Faculty of Pharmaceutical Sciences, Federal University of Alfenas, 37130-001 Alfenas, MG, Brazil., Dias MVS; Institute of Natural Sciences, Federal University of Alfenas, 37130-001 Alfenas, MG, Brazil., Figueiredo EC; Laboratory of Toxicant and Drug Analyses, Faculty of Pharmaceutical Sciences, Federal University of Alfenas, 37130-001 Alfenas, MG, Brazil. Electronic address: eduardo.figueiredo@unifal-mg.edu.br.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Jun; Vol. 269 (Pt 1), pp. 131962. Date of Electronic Publication: 2024 Apr 29.
DOI: 10.1016/j.ijbiomac.2024.131962
Abstrakt: Carbon nanotubes are promising materials for biomedical applications like delivery systems and tissue scaffolds. In this paper, magnetic carbon nanotubes (M-CNTs) covered with bovine serum albumin (M-CNTs-BSA) or functionalized with hydrophilic monomers (M-CNTs-HL) were synthesized, characterized, and evaluated concerning their interaction with Caco-2 cells. There is no comparison between these two types of functionalization, and this study aimed to verify their influence on the material's interaction with the cells. Different concentrations of the nanotubes were applied to investigate cytotoxicity, cell metabolism, oxidative stress, apoptosis, and capability to cross biomimetic barriers. The materials showed cytocompatibility up to 100 μg mL -1 and a hemolysis rate below 2 %. Nanotubes' suspensions were allowed to permeate Caco-2 monolayers for up to 8 h under the effect of the magnetic field. Magnetic nanoparticles associated with the nanotubes allowed estimation of permeation through the monolayers, with values ranging from 0.50 to 7.19 and 0.27 to 9.30 × 10 -3  μg (equivalent to 0.43 to 6.22 and 0.23 to 9.54 × 10 -2  % of the initially estimated mass of magnetic nanoparticles) for cells exposed and non-exposed to the magnets, respectively. Together, these results support that the developed materials are promising for applications in biomedical and biotechnological fields.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Eduardo Costa Figueiredo reports financial support was provided by Federal University of Alfenas. Mariana Azevedo Rosa reports financial support was provided by Coordination of Higher Education Personnel Improvement. Eduardo Costa Figueiredo reports financial support was provided by Minas Gerais State Foundation of Support to the Research. Eduardo Costa Figueiredo reports financial support was provided by National Council for Scientific and Technological Development. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
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