Recombinant cellobiose dehydrogenase from Thermothelomyces thermophilus: Its functional characterization and applicability in cellobionic acid production.

Autor: Oliva B; Department of Biotechnology, Lorena School of Engineering, University of São Paulo, Lorena, SP, Brazil., Velasco J; Department of Biotechnology, Lorena School of Engineering, University of São Paulo, Lorena, SP, Brazil; Biological Sciences Department, Universidad de los Andes, Bogotá DC, Colombia., Leila Berto G; Department of Biotechnology, Lorena School of Engineering, University of São Paulo, Lorena, SP, Brazil., Polikarpov I; São Carlos Institute of Physics, University of São Paulo, São Carlos, SP, Brazil., Cristante de Oliveira L; Department of Physics, Institute of Biosciences, Humanities and Exact Sciences (IBILCE) São Paulo State University 'Júlio de Mesquita Filho' - Unesp - São José do Rio Preto, SP, Brazil., Segato F; Department of Biotechnology, Lorena School of Engineering, University of São Paulo, Lorena, SP, Brazil. Electronic address: segato@usp.br.
Jazyk: angličtina
Zdroj: Bioresource technology [Bioresour Technol] 2024 Jun; Vol. 402, pp. 130763. Date of Electronic Publication: 2024 Apr 30.
DOI: 10.1016/j.biortech.2024.130763
Abstrakt: The fungus Thermothelomyces thermophilus is a thermotolerant microorganism that has been explored as a reservoir for enzymes (hydrolytic enzymes and oxidoreductases). The functional analysis of a recombinant cellobiose dehydrogenase (MtCDHB) from T. thermophilus demonstrated a thermophilic behavior, an optimal pH in alkaline conditions for inter-domain electron transfer, and catalytic activity on cellooligosaccharides with different degree of polymerization. Its applicability was evaluated to the sustainable production of cellobionic acid (CBA), a potential pharmaceutical and cosmetic ingredient rarely commercialized. Dissolving pulp was used as a disaccharide source for MtCDHB. Initially, recombinant exoglucanases (MtCBHI and MtCBHII) from T. thermophilus hydrolyzed the dissolving pulp, resulting in 87% cellobiose yield, which was subsequently converted into CBA by MtCDHB, achieving a 66% CBA yield after 24 h. These findings highlight the potential of MtCDHB as a novel approach to obtaining CBA through the bioconversion of a plant-based source.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: