Analgesic candidate adenosine A 3 receptors are expressed by perineuronal peripheral macrophages in human dorsal root ganglion and spinal cord microglia.

Autor:	Sapio MR; Department of Perioperative Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, United States., Staedtler ES; Department of Perioperative Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, United States., King DM; Department of Perioperative Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, United States., Maric D; National Institute of Neurological Disorders and Stroke, Flow and Imaging Cytometry Core Facility, Bethesda, MD, United States., Jahanipour J; National Institute of Neurological Disorders and Stroke, Flow and Imaging Cytometry Core Facility, Bethesda, MD, United States., Ghetti A; AnaBios Corporation, San Diego, CA, United States., Jacobson KA; National Institute of Diabetes and Digestive and Kidney Diseases, Molecular Recognition Section, Laboratory of Bioorganic Chemistry, Bethesda, MD, United States., Mannes AJ; Department of Perioperative Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, United States., Iadarola MJ; Department of Perioperative Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, United States.
Jazyk:	angličtina
Zdroj:	Pain [Pain] 2024 Oct 01; Vol. 165 (10), pp. 2323-2343. Date of Electronic Publication: 2024 Apr 30.
DOI:	10.1097/j.pain.0000000000003242
Abstrakt:	Abstract: Adenosine receptors are a family of purinergic G protein-coupled receptors that are widely distributed in bodily organs and in the peripheral and central nervous systems. Recently, antihyperalgesic actions have been suggested for the adenosine A 3 receptor, and its agonists have been proposed as new neuropathic pain treatments. We hypothesized that these receptors may be expressed in nociceptive primary afferent neurons. However, RNA sequencing across species, eg, rat, mouse, dog, and human, suggests that dorsal root ganglion (DRG) expression of ADORA3 is inconsistent. In rat and mouse, Adora3 shows very weak to no expression in DRG, whereas it is well expressed in human DRG. However, the cell types in human DRG that express ADORA3 have not been delineated. An examination of DRG cell types using in situ hybridization clearly detected ADORA3 transcripts in peripheral macrophages that are in close apposition to the neuronal perikarya but not in peripheral sensory neurons. By contrast, ADORA1 was found primarily in neurons, where it is broadly expressed at low levels. These results suggest that a more complex or indirect mechanism involving modulation of macrophage and/or microglial cells may underlie the potential analgesic action of adenosine A 3 receptor agonism. (Copyright © 2024 Written work prepared by employees of the Federal Government as part of their official duties is, under the U.S. Copyright Act, a “work of the United States Government” for which copyright protection under Title 17 of the United States Code is not available. As such, copyright does not extend to the contributions of employees of the Federal Government.)
Databáze:	MEDLINE
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