Arabinosylation of cell wall extensin is required for the directional response to salinity in roots.

Autor:	Zou Y; Laboratory of Plant Physiology, Wageningen University & Research, 6708 PB Wageningen, the Netherlands.; Plant Cell Biology, Swammerdam Institute for Life Science, Universiteit van Amsterdam, 1090 GE Amsterdam, the Netherlands., Gigli-Bisceglia N; Laboratory of Plant Physiology, Wageningen University & Research, 6708 PB Wageningen, the Netherlands.; Plant Stress Resilience, Institute of Environmental Biology, Utrecht University, 3508 TB Utrecht, the Netherlands., van Zelm E; Laboratory of Plant Physiology, Wageningen University & Research, 6708 PB Wageningen, the Netherlands., Kokkinopoulou P; Laboratory of Plant Physiology, Wageningen University & Research, 6708 PB Wageningen, the Netherlands., Julkowska MM; Boyce Thompson Institute, Ithaca, NY 14853., Besten M; Laboratory of Biochemistry, Wageningen University & Research, 6708 WE Wageningen, the Netherlands., Nguyen TP; Laboratory of Genetics, Wageningen University & Research, 6708 PB Wageningen, the Netherlands., Li H; Laboratory of Plant Physiology, Wageningen University & Research, 6708 PB Wageningen, the Netherlands., Lamers J; Laboratory of Plant Physiology, Wageningen University & Research, 6708 PB Wageningen, the Netherlands., de Zeeuw T; Laboratory of Plant Physiology, Wageningen University & Research, 6708 PB Wageningen, the Netherlands., Dongus JA; Laboratory of Plant Physiology, Wageningen University & Research, 6708 PB Wageningen, the Netherlands., Zeng Y; Laboratory of Plant Physiology, Wageningen University & Research, 6708 PB Wageningen, the Netherlands., Cheng Y; Laboratory of Plant Physiology, Wageningen University & Research, 6708 PB Wageningen, the Netherlands., Koevoets IT; Laboratory of Plant Physiology, Wageningen University & Research, 6708 PB Wageningen, the Netherlands.; Plant Cell Biology, Swammerdam Institute for Life Science, Universiteit van Amsterdam, 1090 GE Amsterdam, the Netherlands., Jørgensen B; Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg C 1871, Denmark., Giesbers M; Wageningen Electron Microscopy Centre, Wageningen University & Research, 6708 PB Wageningen, the Netherlands., Vroom J; Wageningen Electron Microscopy Centre, Wageningen University & Research, 6708 PB Wageningen, the Netherlands., Ketelaar T; Laboratory of Cell Biology, Wageningen University & Research, 6708 PB Wageningen, the Netherlands., Petersen BL; Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg C 1871, Denmark., Engelsdorf T; Molecular Plant Physiology, Philipps-Universität Marburg, 35043 Marburg, Germany., Sprakel J; Laboratory of Biochemistry, Wageningen University & Research, 6708 WE Wageningen, the Netherlands., Zhang Y; Laboratory of Plant Physiology, Wageningen University & Research, 6708 PB Wageningen, the Netherlands.; College of Agriculture, South China Agricultural University, 510642 Guangzhou, China., Testerink C; Laboratory of Plant Physiology, Wageningen University & Research, 6708 PB Wageningen, the Netherlands.
Jazyk:	angličtina
Zdroj:	The Plant cell [Plant Cell] 2024 Sep 03; Vol. 36 (9), pp. 3328-3343.
DOI:	10.1093/plcell/koae135
Abstrakt:	Soil salinity is a major contributor to crop yield losses. To improve our understanding of root responses to salinity, we developed and exploited a real-time salt-induced tilting assay. This assay follows root growth upon both gravitropic and salt challenges, revealing that root bending upon tilting is modulated by Na+ ions, but not by osmotic stress. Next, we measured this salt-specific response in 345 natural Arabidopsis (Arabidopsis thaliana) accessions and discovered a genetic locus, encoding the cell wall-modifying enzyme EXTENSIN ARABINOSE DEFICIENT TRANSFERASE (ExAD) that is associated with root bending in the presence of NaCl (hereafter salt). Extensins are a class of structural cell wall glycoproteins known as hydroxyproline (Hyp)-rich glycoproteins, which are posttranslationally modified by O-glycosylation, mostly involving Hyp-arabinosylation. We show that salt-induced ExAD-dependent Hyp-arabinosylation influences root bending responses and cell wall thickness. Roots of exad1 mutant seedlings, which lack Hyp-arabinosylation of extensin, displayed increased thickness of root epidermal cell walls and greater cell wall porosity. They also showed altered gravitropic root bending in salt conditions and a reduced salt-avoidance response. Our results suggest that extensin modification via Hyp-arabinosylation is a unique salt-specific cellular process required for the directional response of roots exposed to salinity. Competing Interests: Conflict of interest statement. None declared. (© The Author(s) 2024. Published by Oxford University Press on behalf of American Society of Plant Biologists.)
Databáze:	MEDLINE
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