Magnetic Modulation of Biochemical Synthesis in Synthetic Cells.

Autor:	Zhu KK; Department of Chemistry, Imperial College London, Molecular Sciences Research Hub, White City, London W12 0BZ, U.K.; Department of Chemical Engineering, Imperial College London, South Kensington, London SW7 2AZ, U.K.; fabriCELL, Imperial College London, Molecular Sciences Research Hub, White City, London W12 0BZ, U.K.; Institute of Chemical Biology, Imperial College London, Molecular Sciences Research Hub, White City, London W12 0BZ, U.K., Gispert Contamina I; Department of Chemical Engineering, Imperial College London, South Kensington, London SW7 2AZ, U.K.; fabriCELL, Imperial College London, Molecular Sciences Research Hub, White City, London W12 0BZ, U.K., Ces O; Department of Chemistry, Imperial College London, Molecular Sciences Research Hub, White City, London W12 0BZ, U.K.; fabriCELL, Imperial College London, Molecular Sciences Research Hub, White City, London W12 0BZ, U.K.; Institute of Chemical Biology, Imperial College London, Molecular Sciences Research Hub, White City, London W12 0BZ, U.K., Barter LMC; Department of Chemistry, Imperial College London, Molecular Sciences Research Hub, White City, London W12 0BZ, U.K.; Institute of Chemical Biology, Imperial College London, Molecular Sciences Research Hub, White City, London W12 0BZ, U.K., Hindley JW; Department of Chemistry, Imperial College London, Molecular Sciences Research Hub, White City, London W12 0BZ, U.K.; fabriCELL, Imperial College London, Molecular Sciences Research Hub, White City, London W12 0BZ, U.K.; Institute of Chemical Biology, Imperial College London, Molecular Sciences Research Hub, White City, London W12 0BZ, U.K., Elani Y; Department of Chemical Engineering, Imperial College London, South Kensington, London SW7 2AZ, U.K.; fabriCELL, Imperial College London, Molecular Sciences Research Hub, White City, London W12 0BZ, U.K.
Jazyk:	angličtina
Zdroj:	Journal of the American Chemical Society [J Am Chem Soc] 2024 May 15; Vol. 146 (19), pp. 13176-13182. Date of Electronic Publication: 2024 May 01.
DOI:	10.1021/jacs.4c00845
Abstrakt:	Synthetic cells can be constructed from diverse molecular components, without the design constraints associated with modifying 'living' biological systems. This can be exploited to generate cells with abiotic components, creating functionalities absent in biology. One example is magnetic responsiveness, the activation and modulation of encapsulated biochemical processes using a magnetic field, which is absent from existing synthetic cell designs. This is a critical oversight, as magnetic fields are uniquely bio-orthogonal, noninvasive, and highly penetrative. Here, we address this by producing artificial magneto-responsive organelles by coupling thermoresponsive membranes with hyperthermic Fe 3 O 4 nanoparticles and embedding them in synthetic cells. Combining these systems enables synthetic cell microreactors to be built using a nested vesicle architecture, which can respond to alternating magnetic fields through in situ enzymatic catalysis. We also demonstrate the modulation of biochemical reactions by using different magnetic field strengths and the potential to tune the system using different lipid compositions. This platform could unlock a wide range of applications for synthetic cells as programmable micromachines in biomedicine and biotechnology.
Databáze:	MEDLINE
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