Suppression of the Escherichia coli rnpA49 conditionally lethal phenotype by different compensatory mutations.

Autor:	Babina AM; Department of Medical Biochemistry and Microbiology arianne.babina@imbim.uu.se arianne.babina@glasgow.ac.uk., Kirsebom LA; Department of Cell and Molecular Biology, Uppsala University, 751 23 Uppsala, Sweden., Andersson DI; Department of Medical Biochemistry and Microbiology.
Jazyk:	angličtina
Zdroj:	RNA (New York, N.Y.) [RNA] 2024 Jul 16; Vol. 30 (8), pp. 977-991. Date of Electronic Publication: 2024 Jul 16.
DOI:	10.1261/rna.079909.123
Abstrakt:	RNase P is an essential enzyme found across all domains of life that is responsible for the 5'-end maturation of precursor tRNAs. For decades, numerous studies have sought to elucidate the mechanisms and biochemistry governing RNase P function. However, much remains unknown about the regulation of RNase P expression, the turnover and degradation of the enzyme, and the mechanisms underlying the phenotypes and complementation of specific RNase P mutations, especially in the model bacterium, Escherichia coli In E. coli , the temperature-sensitive (ts) rnpA49 mutation in the protein subunit of RNase P has arguably been one of the most well-studied mutations for examining the enzyme's activity in vivo. Here, we report for the first time naturally occurring temperature-resistant suppressor mutations of E. coli strains carrying the rnpA49 allele. We find that rnpA49 strains can partially compensate the ts defect via gene amplifications of either RNase P subunit ( rnpA49 or rnpB ) or by the acquisition of loss-of-function mutations in Lon protease or RNase R. Our results agree with previous plasmid overexpression and gene deletion complementation studies, and importantly suggest the involvement of Lon protease in the degradation and/or regulatory pathway(s) of the mutant protein subunit of RNase P. This work offers novel insights into the behavior and complementation of the rnpA49 allele in vivo and provides direction for follow-up studies regarding RNase P regulation and turnover in E. coli . (© 2024 Babina et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.)
Databáze:	MEDLINE
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