Design, synthesis and application of dual-channel fluorescent probes for ratiometric detection of HClO and H 2 S based on phenothiazine coumarins.

Autor: Teng Z; Green Catalysis Center, College of Chemistry, Zhengzhou University, Zhengzhou 450001, Henan Province, China., Shangguan H; Green Catalysis Center, College of Chemistry, Zhengzhou University, Zhengzhou 450001, Henan Province, China; College of Ecology and Environment, Zhengzhou University, Zhengzhou 450001, Henan Province, China., Liu L; Green Catalysis Center, College of Chemistry, Zhengzhou University, Zhengzhou 450001, Henan Province, China., Zhang S; Shandong Yiborun New Material Technology Co., Ltd., Binzhou, Shandong Province, China., Li G; Green Catalysis Center, College of Chemistry, Zhengzhou University, Zhengzhou 450001, Henan Province, China., Cheng Z; Green Catalysis Center, College of Chemistry, Zhengzhou University, Zhengzhou 450001, Henan Province, China., Qi F; College of Materials & Chemical Engineering, Hunan City University, Yiyang 413000, China., Liu X; Green Catalysis Center, College of Chemistry, Zhengzhou University, Zhengzhou 450001, Henan Province, China. Electronic address: xingjiangliu@zzu.edu.cn.
Jazyk: angličtina
Zdroj: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2024 Aug 05; Vol. 316, pp. 124312. Date of Electronic Publication: 2024 Apr 18.
DOI: 10.1016/j.saa.2024.124312
Abstrakt: The ubiquity of diverse material entities in environmental matrices renders the deployment of unifunctional fluorescent indicators inadequate. Consequently, this study introduces a ratiometric dual-emission fluorescent sensor (Probe CP), synthesized by conjugating phenothiazine coumarin to hydroxycoumarin through a piperazine linker for concurrent detection of HClO and H 2 S. Upon interaction with HClO, the phenothiazine unit's sulfur atom undergoes oxidation to sulfoxide, facilitating a shift from red to green fluorescence in a ratiometric manner. Concurrently, at the opposite terminus of Probe CP, 2,4-dinitroanisole serves as the reactive moiety for H 2 S recognition; it restores the blue emission characteristic of 7-hydroxycoumarin while maintaining the red fluorescence emanating from phenothiazine coumarin as an internal standard for ratio-based assessment. Exhibiting elevated specificity and sensitivity coupled with minimal detection thresholds (0.0506 μM for HClO and 1.7292 μM for H 2 S) alongside rapid equilibration periods (3 min for HClO and half an hour for H 2 S), this sensor was efficaciously employed in cellular environments and within zebrafish models as well as imaging applications pertaining to alcohol-induced hepatic injury in murine subjects.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: