| Autor: |
Huynh GT; Department of Chemical and Biological Engineering, Monash University, Clayton, VIC 3800, Australia.; Commonwealth Scientific and Industrial Research Organization (CSIRO) Manufacturing, Clayton, VIC 3168, Australia., Tunny SS; Department of Chemical and Biological Engineering, Monash University, Clayton, VIC 3800, Australia., Frith JE; Department of Materials Science and Engineering, Monash University, Clayton, VIC 3800, Australia.; Australian Regenerative Medicine Institute, Monash University, Clayton, VIC 3800, Australia.; ARC Training Centre for Cell and Tissue Engineering Technologies, Monash University, Clayton, VIC 3800, Australia., Meagher L; Department of Materials Science and Engineering, Monash University, Clayton, VIC 3800, Australia.; Australian Regenerative Medicine Institute, Monash University, Clayton, VIC 3800, Australia.; ARC Training Centre for Cell and Tissue Engineering Technologies, Monash University, Clayton, VIC 3800, Australia., Corrie SR; Department of Chemical and Biological Engineering, Monash University, Clayton, VIC 3800, Australia.; ARC Training Centre for Cell and Tissue Engineering Technologies, Monash University, Clayton, VIC 3800, Australia. |
| Abstrakt: |
Oxygen plays a central role in aerobic metabolism, and while many approaches have been developed to measure oxygen concentration in biological environments over time, monitoring spatiotemporal changes in dissolved oxygen levels remains challenging. To address this, we developed a ratiometric core-shell organosilica nanosensor for continuous, real-time optical monitoring of oxygen levels in biological environments. The nanosensors demonstrate good steady state characteristics ( K pSV = 0.40 L/mg, R 2 = 0.95) and respond reversibly to changes in oxygen concentration in buffered solutions and report similar oxygen level changes in response to bacterial cell growth ( Escherichia coli ) in comparison to a commercial bulk optode-based sensing film. We further demonstrated that the oxygen nanosensors could be distributed within a growing culture of E. coli and used to record oxygen levels over time and in different locations within a static culture, opening the possibility of spatiotemporal monitoring in complex biological systems. |
