Characterization and antitumor effect of doxorubicin-loaded Fe 3 O 4 -Au nanocomposite synthesized by electron beam evaporation for magnetic nanotheranostics.

Autor:	Orel VB; National Cancer Institute Kyiv 03022 Ukraine orel.valeriy@gmail.com.; National Technical University of Ukraine 'Igor Sikorsky Kyiv Polytechnic Institute' Kyiv 03056 Ukraine., Kurapov YA; E.O. Paton Electric Welding Institute Kyiv 03150 Ukraine., Lytvyn SY; E.O. Paton Electric Welding Institute Kyiv 03150 Ukraine., Orel VE; National Cancer Institute Kyiv 03022 Ukraine orel.valeriy@gmail.com.; National Technical University of Ukraine 'Igor Sikorsky Kyiv Polytechnic Institute' Kyiv 03056 Ukraine., Galkin OY; National Technical University of Ukraine 'Igor Sikorsky Kyiv Polytechnic Institute' Kyiv 03056 Ukraine., Dasyukevich OY; National Cancer Institute Kyiv 03022 Ukraine orel.valeriy@gmail.com., Rykhalskyi OY; National Cancer Institute Kyiv 03022 Ukraine orel.valeriy@gmail.com., Diedkov AG; National Cancer Institute Kyiv 03022 Ukraine orel.valeriy@gmail.com., Ostafiichuk VV; National Cancer Institute Kyiv 03022 Ukraine orel.valeriy@gmail.com., Lyalkin SA; National Cancer Institute Kyiv 03022 Ukraine orel.valeriy@gmail.com., Burlaka AP; R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology Kyiv 03022 Ukraine., Virko SV; R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology Kyiv 03022 Ukraine.; V.E. Lashkaryov Institute of Semiconductor Physics Kyiv 03028 Ukraine., Skoryk MA; G.V. Kurdyumov Institute for Metal Physics of the N.A.S. of Ukraine Kyiv 03142 Ukraine., Zagorodnii VV; National Technical University of Ukraine 'Igor Sikorsky Kyiv Polytechnic Institute' Kyiv 03056 Ukraine.; G.V. Kurdyumov Institute for Metal Physics of the N.A.S. of Ukraine Kyiv 03142 Ukraine., Stelmakh YA; E.O. Paton Electric Welding Institute Kyiv 03150 Ukraine., Didikin GG; E.O. Paton Electric Welding Institute Kyiv 03150 Ukraine., Oranska OI; Chuiko Institute of Surface Chemistry of the N.A.S. of Ukraine Kyiv 03164 Ukraine., Calcagnile L; University of Salento Lecce 73100 Italy., Manno DE; University of Salento Lecce 73100 Italy., Rinaldi R; University of Salento Lecce 73100 Italy., Nedostup YV; Taras Shevchenko National University of Kyiv Kyiv 03680 Ukraine.
Jazyk:	angličtina
Zdroj:	RSC advances [RSC Adv] 2024 Apr 29; Vol. 14 (20), pp. 14126-14138. Date of Electronic Publication: 2024 Apr 29 (Print Publication: 2024).
DOI:	10.1039/d4ra01777c
Abstrakt:	Magnetic nanocomposites (MNC) are promising theranostic platforms with tunable physicochemical properties allowing for remote drug delivery and multimodal imaging. Here, we developed doxorubicin-loaded Fe 3 O 4 -Au MNC (DOX-MNC) using electron beam physical vapor deposition (EB-PVD) in combination with magneto-mechanochemical synthesis to assess their antitumor effect on Walker-256 carcinosarcoma under the influence of a constant magnetic (CMF) and electromagnetic field (EMF) by comparing tumor growth kinetics, magnetic resonance imaging (MRI) scans and electron spin resonance (ESR) spectra. Transmission (TEM) and scanning electron microscopy (SEM) confirmed the formation of spherical magnetite nanoparticles with a discontinuous gold coating that did not significantly affect the ferromagnetic properties of MNC, as measured by vibrating-sample magnetometry (VSM). Tumor-bearing animals were divided into the control (no treatment), conventional doxorubicin (DOX), DOX-MNC and DOX-MNC + CMF + EMF groups. DOX-MNC + CMF + EMF resulted in 14% and 16% inhibition of tumor growth kinetics as compared with DOX and DOX-MNC, respectively. MRI visualization showed more substantial tumor necrotic changes after the combined treatment. Quantitative analysis of T 2 -weighted (T 2 W) images revealed the lowest value of skewness and a significant increase in tumor intensity in response to DOX-MNC + CMF + EMF as compared with the control (1.4 times), DOX (1.6 times) and DOX-MNC (1.8 times) groups. In addition, the lowest level of nitric oxide determined by ESR was found in DOX-MNC + CMF + EMF tumors, which was close to that of the muscle tissue in the contralateral limb. We propose that the reason for the relationship between the observed changes in MRI and ESR is the hyperfine interaction of nuclear and electron spins in mitochondria, as a source of free radical production. Therefore, these results point to the use of EB-PVD and magneto-mechanochemically synthesized Fe 3 O 4 -Au MNC loaded with DOX as a potential candidate for cancer magnetic nanotheranostic applications. Competing Interests: Authors declare no conflicts of interest. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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