Isotope Distribution Analysis in H₂ 18 O Pulse-Labeled Trees Frozen with Liquid Nitrogen.

Autor: Xiang Y; Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan., Kagawa A; Forestry and Forest Products Research Institute, Wood Anatomy and Quality Laboratory, Ibaraki, Japan., Nagai S; Forestry Technology Institute, Hyogo Prefectural Technology Center for Agriculture, Forestry and Fisheries, Hyogo, Japan., Yasuda Y; Department of Environmental Sciences and Technology, Faculty of Agriculture, Kagoshima University, Kagoshima City Kagoshima, Japan., Utsumi Y; Kyushu University Forest, Kyushu University, Fukuoka, Japan.
Jazyk: angličtina
Zdroj: Physiologia plantarum [Physiol Plant] 2024 May-Jun; Vol. 176 (3), pp. e14292.
DOI: 10.1111/ppl.14292
Abstrakt: Tracer injection has long been recognized as a valuable tool for delineating tree hydraulics and assessing water transport pathways. Recently, isotope tracers have emerged as innovative instruments for investigating tree hydraulics, providing new insights into tree water dynamics. Nevertheless, there is a critical need for further research to comprehensively grasp water movement and distribution within trees. A previously introduced technique for analyzing the isotopic ratio of water in wet tissues, offering millimeter-scale resolution for visualizing tracer movement, faces challenges due to its underdeveloped sample preparation techniques. In this study, we introduced an H 2 18 O tracer into S. gracilistyla samples, exclusively comprising indeterminate roots, stems, and leaves, cultivated through hydroponics and grown within the current year. Our objective was to assess the axial distribution of the tracer in the xylem. Additionally, we devised a novel method for preparing frozen wet tissue samples, enhancing the repeatability and success rate of experiments. The results demonstrated that all frozen wet tissue samples exhibited an average water loss rate of less than 0.6%. Isotopic analysis of these samples unveiled a consistent decline in tracer concentration with increasing height in all Salix specimens, with three out of five samples revealing a significant isotope gradient. Our findings affirm the efficacy and practicality of combining isotopic labeling with freezing, stabilization, and preparation techniques. Looking ahead, our isotopic labeling and analysis methods are poised to transcend woody plants, finding extensive applications in plant physiology and ecohydrology.
(© 2024 Scandinavian Plant Physiology Society.)
Databáze: MEDLINE
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