Combination of 308-nm excimer laser and piperine promotes melanocyte proliferation, migration, and melanin content production via the miR-328/SFRP1 axis.

Autor: Wu Y; Department of Dermatology, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China., Zhang J; Department of Dermatology, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China., Du S; Department of Laboratory, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China., Wang X; Department of Dermatology, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China., Li J; Department of Dermatology, Traditional Chinese Medicine Hospital of Jinggu County, Puer, Yunnan, China., Chen Y; Department of Dermatology, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China., Zhou H; Department of Dermatology, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China., Gao S; Department of Dermatology, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China., Li Y; Department of Dermatology, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China., Liu X; Department of Rheumatology and Clinical Immunology, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China.
Jazyk: angličtina
Zdroj: Photodermatology, photoimmunology & photomedicine [Photodermatol Photoimmunol Photomed] 2024 May; Vol. 40 (3), pp. e12970.
DOI: 10.1111/phpp.12970
Abstrakt: Objective: Both piperine and a 308-nm excimer laser have significant curative effects on vitiligo. This study mainly explored the molecular mechanism of a 308-nm excimer combined with piperine in regulating melanocyte proliferation.
Methods: Epidermal melanocytes were cultured in piperine solution, and the cells were irradiated by an XTRAC excimer laser treatment system at 308-nm output monochromatic light. Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot were for detecting the expression levels of genes or proteins. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and Transwell method was for assessing cell viability and migration capacity. The content of melanin was also detected.
Results: The combination of the 308-nm excimer laser and piperine enhanced the cell proliferation, migration, and melanin production of melanocytes and upregulated the level of miR-328, and restraint of miR-328 reversed the influence of the 308-nm excimer laser and piperine. Secreted frizzled-related protein 1 (SFRP1) is a direct target gene of miR-328, and miR-328 can inhibit the expression of SFRP1 and elevate the protein level of the Wnt/β-catenin signaling pathway.
Conclusion: The 308-nm excimer laser combined with piperine may be more efficient than piperine alone in the remedy of vitiligo, and the miR-328/SFRP1 and Wnt/β-catenin pathways are participated in the proliferation, migration, and melanin synthesis of melanocytes.
(© 2024 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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