Expression of PVT-1 and miR-29a/29b as reliable biomarkers for liver cirrhosis and their correlation with the inflammatory biomarkers profile.

Autor:	Khalaf SE; Department of Biochemistry, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt., Abdelfattah SN; Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt., Khaliefa AK; Department of Biochemistry, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt., Daoud SA; Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt.; Faculty of Medicine, Beni Suef National University, Beni-Suef, Egypt., Yahia E; Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt.; Faculty of Medicine, Beni Suef National University, Beni-Suef, Egypt., Hasona NA; Department of Biochemistry, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt.; Biochemistry Department, Faculty of Science, Beni Suef National University, Beni-Suef, Egypt.
Jazyk:	angličtina
Zdroj:	Human & experimental toxicology [Hum Exp Toxicol] 2024 Jan-Dec; Vol. 43, pp. 9603271241251451.
DOI:	10.1177/09603271241251451
Abstrakt:	Background & Aims: The liver is a vital organ responsible for numerous metabolic processes, which can be significantly impacted by long non-coding RNAs (lncRNAs) and microRNAs (miRNAs). These ribonucleic acid (RNA) molecules have been shown to play a crucial role in regulating gene expression, and their dysregulation has been implicated in numerous liver disorders. Our study aimed to investigate the diagnostic accuracy of plasmacytoma variant translocation-1 (PVT-1), microRNA-29a/29b (miR-29a/miR-29b), and inflammatory biomarkers [ interleukine-6 (IL-6), tumor necrosis factor-alpha (TNF-α), transforming growth factor-beta (TGF-β), and insulin growth factor-1 (IGF-1)] as diagnostic and prognostic biomarkers for liver cirrhosis. Therefore, understanding the mechanisms by which lncRNAs and miRNAs influence liver metabolism is of paramount importance in developing effective treatments for liver-related diseases. Methods: Serum samples were collected from 164 participants, comprising 114 cirrhotic patients with varying grades (35 grade I, 35 grade II, and 44 grade III) and 50 healthy controls. PVT-1 and miR-29a/miR-29b expression was analyzed by reverse transcription-quantitative polymerase chain reaction (RT-PCR), while the serum levels of inflammatory biomarkers were assessed using enzyme-linked immunosorbent assay (ELISA). Results: The study participants exhibited notable differences in PVT-1 and miR-29a/miR-29b expression. ROC analysis revealed excellent discriminative power for PVT-1 and miR-29a/miR-29b in distinguishing cirrhotic patients from healthy controls. Conclusion: This study demonstrates the promising potential of PVT-1 and miR-29a/miR-29b as early diagnostic biomarkers for liver cirrhosis detection, requiring further validation in larger cohorts. Our findings also reinforce the diagnostic value of circulating inflammatory biomarkers (IL-6, TNF-α, TGF-β, and IGF-1) levels for liver cirrhosis screening. Competing Interests: Declaration of conflicting interestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
Databáze:	MEDLINE
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