Exploring mosquito abundance and Plasmodium infection through nested-PCR: implications for disease surveillance and control.

Autor: Abbas H; Department of Parasitology, Faculty of Veterinary Science, University of Agriculture, Faisalabad, 38040, Pakistan. haider.abbas@uvas.edu.pk.; Department of Pathobiology (Parasitology Section), KBCMA College of Veterinary and Animal Sciences, Narowal, Sub-Campus, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan. haider.abbas@uvas.edu.pk., Sajid MS; Department of Parasitology, Faculty of Veterinary Science, University of Agriculture, Faisalabad, 38040, Pakistan., Rizwan HM; Department of Pathobiology (Parasitology Section), KBCMA College of Veterinary and Animal Sciences, Narowal, Sub-Campus, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan., Tahir UB; Department of Parasitology, Faculty of Veterinary Science, University of Agriculture, Faisalabad, 38040, Pakistan.; Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China., Farooqi SH; Department of Clinical Sciences (Medicine Section), KBCMA College of Veterinary and Animal Sciences, Narowal, Sub-Campus, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan., Iqbal Z; Department of Animal Sciences (Livestock Section), KBCMA College of Veterinary and Animal Sciences, Narowal, Sub-Campus, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan., Malik MA; Department of Parasitology, Faculty of Veterinary Science, University of Agriculture, Faisalabad, 38040, Pakistan., Yaseen K; Institute of Microbiology, University of Agriculture, Faisalabad, 38040, Pakistan., Maqbool M; Department of Parasitology, Faculty of Veterinary Science, University of Agriculture, Faisalabad, 38040, Pakistan., Raza FA; Health Research Institute, National Institute of Health, Research Centre, , King Edward Medical University, Lahore, 54000, Pakistan., Raza M; Department of Basic Sciences (Physiology Section), KBCMA College of Veterinary and Animal Sciences, Narowal, Sub-Campus, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan., Fouad D; Department of Zoology, College of Science, King Saud University, PO Box 22452, Riyadh, 11495, Saudi Arabia., Ataya FS; Department of Biochemistry, College of Science, King Saud University, PO Box 2455, Riyadh, 11451, Saudi Arabia.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Apr 30; Vol. 14 (1), pp. 9871. Date of Electronic Publication: 2024 Apr 30.
DOI: 10.1038/s41598-024-60662-x
Abstrakt: The Plasmodium is responsible for malaria which poses a major health threat, globally. This study is based on the estimation of the relative abundance of mosquitoes, and finding out the correlations of meteorological parameters (temperature, humidity and rainfall) with the abundance of mosquitoes. In addition, this study also focused on the use of nested PCR (species-specific nucleotide sequences of 18S rRNA genes) to explore the Plasmodium spp. in female Anopheles. In the current study, the percentage relative abundance of Culex mosquitoes was 57.65% and Anopheles 42.34% among the study areas. In addition, the highest number of mosquitoes was found in March in district Mandi Bahauddin at 21 °C (T max  = 27, T min  = 15) average temperature, 69% average relative humidity and 131 mm rainfall, and these climatic factors were found to affect the abundance of the mosquitoes, directly or indirectly. Molecular analysis showed that overall, 41.3% of the female Anopheles pools were positive for genus Plasmodium. Among species, the prevalence of Plasmodium (P.) vivax (78.1%) was significantly higher than P. falciparum (21.9%). This study will be helpful in the estimation of future risk of mosquito-borne diseases along with population dynamic of mosquitoes to enhance the effectiveness of vector surveillance and control programs.
(© 2024. The Author(s).)
Databáze: MEDLINE
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