UPLC/Q-TOF-MS-based metabolomics and molecular docking analysis of Bifidobacterium adolescentis exposure to levofloxacin.

Autor: Feng S; Pharmaceutical College, Guangxi Medical University, Nanning, China.; The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China., Guo Y; Pharmaceutical College, Guangxi Medical University, Nanning, China.; School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, China., Wang Q; Department of pharmacy, Guangxi Medical University Cancer Hospital, Nanning, China., Meng M; Pharmaceutical College, Guangxi Medical University, Nanning, China., Liu X; Pharmaceutical College, Guangxi Medical University, Nanning, China., Zhang C; Pharmaceutical College, Guangxi Medical University, Nanning, China., Zheng H; Life Sciences Institute, Guangxi Medical University, Nanning, China., Guo H; Pharmaceutical College, Guangxi Medical University, Nanning, China., Lu R; Guangxi Institute for Food and Drug Control, Nanning, China., Li D; Guangxi Institute for Food and Drug Control, Nanning, China., Su Z; Pharmaceutical College, Guangxi Medical University, Nanning, China., Song H; Pharmaceutical College, Guangxi Medical University, Nanning, China., Liang Y; Pharmaceutical College, Guangxi Medical University, Nanning, China.
Jazyk: angličtina
Zdroj: Biomedical chromatography : BMC [Biomed Chromatogr] 2024 Jun; Vol. 38 (6), pp. e5862. Date of Electronic Publication: 2024 Apr 29.
DOI: 10.1002/bmc.5862
Abstrakt: Antibiotic-associated diarrhea is a common adverse reaction caused by the widespread use of antibiotics. The decrease in probiotics is one of the reasons why antibiotics cause drug-induced diarrhea. However, few studies have addressed the intrinsic mechanism of antibiotics inhibiting probiotics. To investigate the underlying mechanism of levofloxacin against Bifidobacterium adolescentis, we used a metabolomics mass spectrometry-based approach and molecular docking analysis for a levofloxacin-induced B. adolescentis injury model. The results showed that levofloxacin reduced the survival rate of B. adolescentis and decreased the number of B. adolescentis. The untargeted metabolomics analysis identified 27 potential biomarkers, and many of these metabolites are involved in energy metabolism, amino acid metabolism and the lipid metabolism pathway. Molecular docking showed that levofloxacin can bind with aminoacyl-tRNA synthetase and lactic acid dehydrogenase. This result provides a novel insight into the mechanism of the adverse reactions of levofloxacin.
(© 2024 John Wiley & Sons Ltd.)
Databáze: MEDLINE
Externí odkaz: