In vivo assessment of human corneal epithelial cells in orthokeratology lens wearers: A pilot study.
| Autor: | Pattan HF; School of Optometry, Indiana University, Bloomington, Indiana., Liu X; School of Optometry, Indiana University, Bloomington, Indiana., Tankam P |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Optometry and vision science : official publication of the American Academy of Optometry [Optom Vis Sci] 2024 May 01; Vol. 101 (5), pp. 263-271. Date of Electronic Publication: 2024 Apr 25. |
| DOI: | 10.1097/OPX.0000000000002130 |
| Abstrakt: | Significance: Central corneal epithelial thinning associated with midperipheral epithelial thickening has been reported as the main factor contributing to the effectiveness of orthokeratology (ortho-k) in myopia control. Yet, the cellular mechanism governing the regional change in refractive power remains elusive. Purpose: This study aimed to evaluate the correlation between the regional change in corneal epithelial thickness and cell density in ortho-k wearers. Methods: A new human prototype of a polarization-dependent optical coherence microscope was developed to enable noncontact and noninvasive in vivo imaging of corneal epithelial cells in ortho-k wearers with and without their ortho-k lens. The epithelial thickness and cell density were evaluated at the central and midperipheral corneal locations in four ortho-k wearers and four spectacle wearers serving as controls. Results: Polarization-dependent optical coherence microscope achieved in vivo volumetric imaging of all epithelial cell types in ortho-k wearers with and without their lens over a field of view of 0.5 × 0.5 mm 2 with an isotropic resolution of ~2.2 mm. The central epithelial thinning and midperipheral epithelial thickening were consistent across all ortho-k wearers. However, the inconsistency in their regional epithelial cell density highlighted a great variability in individual response to ortho-k treatment. There was no strong correlation between epithelial thickness and cell density, especially at the midperipheral cornea, in ortho-k participants. Conclusions: This study constitutes our first step toward uncovering the cellular mechanism underlying the effectiveness of ortho-k in myopia control. Future studies will focus on the longitudinal evaluation of epithelial cells before and during ortho-k treatment to identify factors governing individual response to ortho-k treatment and ultimately inform the dynamics of epithelial cells taking place during the ortho-k treatment. Competing Interests: Conflict of Interest Disclosure: None of the authors have reported a financial conflict of interest. The sponsor provided financial support but had no role in the study design, conduct, analysis and interpretation, or writing of the report. (Copyright © 2024 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the American Academy of Optometry.) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|