| Autor: |
Ali F; School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), School of Tropical Agriculture and Forestry Hainan University, Sanya 572025, Hai-nan, China; and Department of Botany, University of Baltistan Skardu, Gilgil Baltistan, 16100, Pakistan., Arif MAR; Nuclear Institute for Agriculture and Biology, Faisalabad, Pakistan., Ali A; Department of Plant Sciences, Quaid-I-Azam University, Islamabad, 45320, Pakistan., Nadeem MA; Faculty of Agricultural Sciences and Technologies, Sivas University of Science and Technology, Sivas 58140, Turkey., Aksoy E; Department of Biological Sciences, Middle East Technical University, Ankara, Turkey., Bakhsh A; Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan., Khan SU; Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City and Southwest University, College of Agronomy and Biotechnology, Southwest University, Chongqing, 400715, China; and Women Medical and Dental College, Khyber Medical University, Peshawar, KPK, 22020, Pakistan., Kurt C; Department of Field Crops, Faculty of Agriculture, University of Çukurova, Adana, Turkey., Tekdal D; Faculty of Science, Department of Biotechnology, Mersin University, 33343, Yenisehir, Mersin, Turkey., Ilyas MK; National Agricultural Research Centre, Park Road, Islamabad 45500, Pakistan., Hameed A; Nuclear Institute for Agriculture and Biology, Faisalabad, Pakistan., Chung YS; Department of Plant Resources and Environment, Jeju National University, Jeju 63243, Republic of Korea., Baloch FS; Faculty of Science, Department of Biotechnology, Mersin University, 33343, Yenisehir, Mersin, Turkey. |
| Abstrakt: |
Effective identification and usage of genetic variation are prerequisites for developing nutrient-efficient cultivars. A collection of 94 safflower (Carthamus tinctorius ) genotypes (G) was investigated for important morphological and photosynthetic traits at four nitrogen (N) treatments. We found significant variation for all the studied traits except chlorophyll b (chl b ) among safflower genotypes, nitrogen treatments and G×N interaction. The examined traits showed a 2.82-50.00% increase in response to N application. Biological yield (BY) reflected a significantly positive correlation with fresh shoot weight (FSW), root length (RL), fresh root weight (FRW) and number of leaves (NOL), while a significantly positive correlation was also observed among carotenoids (C), chlorophyll a (chl a ), chl b and total chlorophyll content (CT) under all treatments. Superior genotypes with respect to plant height (PH), FSW, NOL, RL, FRW and BY were clustered into Group 3, while genotypes with better mean performance regarding chl a , chl b C and CT were clustered into Group 2 as observed in principal component analysis. The identified eight best-performing genotypes could be useful to develop improved nitrogen efficient cultivars. Genome-wide association analysis resulted in 32 marker-trait associations (MTAs) under four treatments. Markers namely DArT-45481731 , DArT-17812864 , DArT-15670279 and DArT-45482737 were found consistent. Protein-protein interaction networks of loci associated with MTAs were related to fatty acid and branched-chain amino acid metabolism and histone modifications. |
