Aptamer-aided plasmonic nano-urchins for reporter-free surface-enhanced Raman spectroscopy analysis of cortisol.

Autor: Li C; School of Chemical Science and Technology, National Demonstration Center for Experimental Chemistry and Chemical Engineering Education, Yunnan University, Kunming, Yunnan, 650091, China. huxufang@ynu.edu.cn., Hu J; Clinical Psychology Department, Zhongshan Third People's Hospital, Zhongshan, Guangdong, 528451, China., Hu N; School of Chemical Science and Technology, National Demonstration Center for Experimental Chemistry and Chemical Engineering Education, Yunnan University, Kunming, Yunnan, 650091, China. huxufang@ynu.edu.cn., Zhao J; School of Chemical Science and Technology, National Demonstration Center for Experimental Chemistry and Chemical Engineering Education, Yunnan University, Kunming, Yunnan, 650091, China. huxufang@ynu.edu.cn., Li Q; School of Chemical Science and Technology, National Demonstration Center for Experimental Chemistry and Chemical Engineering Education, Yunnan University, Kunming, Yunnan, 650091, China. huxufang@ynu.edu.cn., Han Y; School of Chemical Science and Technology, National Demonstration Center for Experimental Chemistry and Chemical Engineering Education, Yunnan University, Kunming, Yunnan, 650091, China. huxufang@ynu.edu.cn., Liu Y; School of Chemical Science and Technology, National Demonstration Center for Experimental Chemistry and Chemical Engineering Education, Yunnan University, Kunming, Yunnan, 650091, China. huxufang@ynu.edu.cn., Hu X; School of Chemical Science and Technology, National Demonstration Center for Experimental Chemistry and Chemical Engineering Education, Yunnan University, Kunming, Yunnan, 650091, China. huxufang@ynu.edu.cn., Zheng L; School of Chemical Science and Technology, National Demonstration Center for Experimental Chemistry and Chemical Engineering Education, Yunnan University, Kunming, Yunnan, 650091, China. huxufang@ynu.edu.cn., Cao Q; School of Chemical Science and Technology, National Demonstration Center for Experimental Chemistry and Chemical Engineering Education, Yunnan University, Kunming, Yunnan, 650091, China. huxufang@ynu.edu.cn.
Jazyk: angličtina
Zdroj: Analytical methods : advancing methods and applications [Anal Methods] 2024 May 16; Vol. 16 (19), pp. 3067-3073. Date of Electronic Publication: 2024 May 16.
DOI: 10.1039/d4ay00401a
Abstrakt: Cortisol is a vital glucocorticoid hormone reflecting stress levels and related disease processes. In this study, we report an aptamer-functionalized plasmonic nano-urchin (α-FeOOH@Au-aptamer)-aided cortisol-capturing and surface-enhanced Raman spectroscopy (SERS) analysis approach. The designed α-FeOOH@Au-aptamer exhibits a well-patterned plasma structure, which combines the good SERS enhancement ability of reduced nanogaps between the Au plasma and the hot spot-favored structure of anisotropic tips from α-FeOOH urchins, with the high affinity of the aptamer towards cortisol molecules. The α-FeOOH@Au-aptamer achieved reporter-free SERS quantification for cortisol with good sensitivity (limit of detection <0.28 μmol L -1 ), robust salt (1.0 mol per L NaCl) and protein (5.0 mg per mL bovine serum protein) tolerance, favorable reproducibility, as well as good reusability. We further demonstrated the good cortisol-capturing ability and SERS efficacy of the α-FeOOH@Au-aptamer profiling in the serum and urine samples. Our approach provides an alternative tool for cortisol analysis and a reference strategy for report-free SERS detection of small molecules.
Databáze: MEDLINE
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