ddRAD Sequencing and DNA Barcoding.
| Autor: | Ivanov V; Ecology and Genetics Research Unit, University of Oulu, Oulu, Finland., Lee KM; Zoology Unit, Finnish Museum of Natural History, University of Helsinki, Helsinki, Finland. kyung.m.lee@helsinki.fi., Mutanen M; Ecology and Genetics Research Unit, University of Oulu, Oulu, Finland. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2744, pp. 213-221. |
| DOI: | 10.1007/978-1-0716-3581-0_13 |
| Abstrakt: | Double-digest restriction site-associated DNA sequencing is a library preparation protocol that enables capturing variable sites across the genome including single-nucleotide polymorphisms (SNPs). These SNPs can be utilized to gain evolutionary insights into patterns observed in DNA barcodes, to infer population structure and phylogenies, to detect gene flow and introgression, and to perform species delimitation analyses. The protocol includes chemically shearing genomic DNA with restriction enzymes, unique tagging, size selection, and amplification of the resulting DNA fragments. Here we provide a detailed description of each step of the protocol, as well as information on essential equipment and common issues encountered during laboratory work. (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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