[IgE reactivity of sera from patients with autoimmune diseases against a multi-epitope protein constructed from T epitopes of Ascaris lumbricoides allergens].

Autor: Fang L; Universidad del Norte. División Ciencias de la Salud, Departamento de Medicina, Barranquilla, Colombia. Lfang@uninorte.edu.co., Martínez D; Universidad del Norte. División Ciencias de la Salud, Departamento de Medicina, Barranquilla, Colombia.; Universidad de Cartagena. Instituto de Investigaciones Inmunológicas, Cartagena, Colombia., Meza-Torres C; Universidad del Norte. División Ciencias de la Salud, Departamento de Medicina, Barranquilla, Colombia.; Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide, Sevilla, España., Moreno-Woo A; Universidad del Norte. División Ciencias de la Salud, Departamento de Medicina, Barranquilla, Colombia., Pereira-Sanandres N; Universidad del Norte. División Ciencias de la Salud, Departamento de Medicina, Barranquilla, Colombia.; Universidad Simón Bolívar, Facultad de Ciencias de la Salud, Barranquilla, Colombia., Domínguez-Vargas A; Universidad del Norte. División Ciencias de la Salud, Departamento de Medicina, Barranquilla, Colombia.; Universidad Simón Bolívar, Facultad de Ciencias de la Salud, Barranquilla, Colombia., Garavito G; Universidad del Norte. División Ciencias de la Salud, Departamento de Medicina, Barranquilla, Colombia.; Universidad Simón Bolívar, Facultad de Ciencias de la Salud, Barranquilla, Colombia., Egea E; Universidad del Norte. División Ciencias de la Salud, Departamento de Medicina, Barranquilla, Colombia.; Universidad Simón Bolívar, Facultad de Ciencias de la Salud, Barranquilla, Colombia.
Jazyk: Spanish; Castilian
Zdroj: Revista alergia Mexico (Tecamachalco, Puebla, Mexico : 1993) [Rev Alerg Mex] 2024 Feb 01; Vol. 71 (1), pp. 53. Date of Electronic Publication: 2024 Feb 01.
DOI: 10.29262/ram.v71i1.1366
Abstrakt: Objective: To evaluate the IgE reactivity of sera in patients suffering from type 1 diabetes (T1D), lupus nephritis (LN) and juvenile idiopathic arthritis (JIA) against a molecule constructed from T epitopes of A. lumbricoides allergens.
Methods: We designed and expressed a synthetic multi-epítope protein named MP1 from A. lumbricoides and house dust mites allergens. By indirect ELISA, we evaluated IgE-reactivity to MP1 and to the whole-body extract of Ascaris lumbricoides in 45 sera from Colombian Caribbean patients with lupus nephritis (LN; n=25), type 1 diabetes (T1D; n=10) and Juvenil idiopathic arthritis (JIA; n=10). Individuals with poly autoimmunity were excluded. All patients were referred to the study by their specialist doctor.
Results: IgE to whole-body extract of A. lumbricoides showed the following median concentrations.
484.2 ng/ml (IQR: 203.4) in JIA patients, 325.6 ng/ml (IQR: 179.3) in individuals with LN, and 424.7 ng/ml (IQR: 80.1) in the T1D group. On the other hand, IgE-reactivity to MP1 was 126.4 ng/ml (IQR: 90.9) in JIA patients, 130.7 ng/ml (IQR: 94.8) in an individual with LN, and 148.8 ng/ml (IQR: 102.1) in the T1D group. Although no statistical differences were observed between patient groups, the IgE to MP1 in all patients (n: 45) (IgE median: 134.2 ng/ml; IQR: 100) were significantly less compared to Ascaris extract (IgE median: 380.7 ng/ml; IQR: 175.8); ( W: 0.732; p-value: 1.034x10 -7 ).
Conclusions: These preliminary results suggest that MP1 showed antigenic properties with low IgE- reactivity, compared to Ascaris lumbricoides extracted in individuals with autoimmune diseases. Further studies are needed to understand better the immune response induced by this molecule.
Databáze: MEDLINE
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