Antimicrobial resistance patterns and genes of Campylobacter jejuni isolated from chickens in Pasuruan, Indonesia.
| Autor: | Yanestria SM; Doctoral Program in Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia., Effendi MH; Division of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia., Tyasningsih W; Division of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia., Moses IB; Department of Applied Microbiology, Faculty of Science, Ebonyi State University, Abakaliki, Nigeria., Khairullah AR; Research Center for Veterinary Science, National Research and Innovation Agency (BRIN), Bogor, Indonesia., Kurniawan SC; Master Program of Animal Sciences, Department of Animal Sciences, Specialisation in Molecule, Cell and Organ Functioning, Wageningen University and Research, Wageningen, The Netherlands., Dameanti FNAEP; Microbiology and Immunology Laboratory, Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, Indonesia., Ikaratri R; Bacteriology Laboratory, Balai Besar Veteriner Wates, Yogyakarta, Indonesia., Pratama JWA; Doctoral Program in Veterinary Science, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia., Sigit M; Division of Animal Husbandry, Faculty of Veterinary Medicine, Universitas Wijaya Kusuma Surabaya, Surabaya, Indonesia., Hasib A; School of Agriculture and Food Sustainability, The University of Queensland, Gatton, Australia., Silaen OSM; Doctoral Program in Biomedical Science, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia. |
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| Jazyk: | angličtina |
| Zdroj: | Open veterinary journal [Open Vet J] 2024 Mar; Vol. 14 (3), pp. 759-768. Date of Electronic Publication: 2024 Mar 31. |
| DOI: | 10.5455/OVJ.2024.v14.i3.2 |
| Abstrakt: | Background: Poultry is one of the most prominent sources of Campylobacter jejuni , which is also a major means of transmission to people. Campylobacter jejuni contamination in chicken meat comes from chicken feces because it naturally exists in the intestines of chickens. Aim: The purpose of this study is to identify the antibiotic resistance patterns and genes of C. jejuni , which was found in chickens in Pasuruan, Indonesia. Methods: The samples used in this study were 200 contents of the small intestine of broiler chickens from 40 farms in Pasuruan Regency. The enriched sample was streaked on the selective media of modified charcoal cefoperazone deoxycholate agar containing the CCDA selective supplement. Antimicrobial susceptibility test utilizing the Kirby-Bauer diffusion test method in accordance with Clinical and Laboratory Standards Institute standards. The polymerase chain reaction (PCR) method was used to detect the ( hip O), which encodes the C. jejuni strain, fluoroquinolone resistance ( gyr A ) , beta-lactam resistance ( bla Results: The findings revealed a 14% (28/200) prevalence of C. jejuni in the small intestine of broiler chickens. These isolates showed high resistance to enrofloxacin (92.9%). All isolates (100%) were susceptible to amoxicillin-clavulanate. The PCR results showed all C. jejuni isolates (100%) detected the gyrA gene, 96.4% detected the bla Conclusion: The findings of antimicrobial resistance at a high level from the small intestine of broiler chickens illustrate the potential threat to human health. To lessen the effects now and in the future, coordinated and suitable action is needed, as well as steps to guarantee the poultry industry's economic survival and public health insurance. Competing Interests: The authors declare that there is no conflict of interest. |
| Databáze: | MEDLINE |
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