Development of an Enzyme-Linked Immunosorbent Assay (ELISA) as a tool to detect NS1 of dengue virus serotype 2 in female Aedes aegypti eggs for the surveillance of dengue fever transmission.

Autor:	Argotte-Ramos R; Centro de Investigación Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, México. Av. Universidad 655, C. P. 62100 Cuernavaca, Morelos, Mexico., Cime-Castillo J; Centro de Investigación Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, México. Av. Universidad 655, C. P. 62100 Cuernavaca, Morelos, Mexico., Vargas V; Centro de Investigación Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, México. Av. Universidad 655, C. P. 62100 Cuernavaca, Morelos, Mexico., Lanz-Mendoza H; Centro de Investigación Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, México. Av. Universidad 655, C. P. 62100 Cuernavaca, Morelos, Mexico., Rodriguez MH; Centro de Investigación Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, México. Av. Universidad 655, C. P. 62100 Cuernavaca, Morelos, Mexico., Rodriguez MC; Centro de Investigación Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, México. Av. Universidad 655, C. P. 62100 Cuernavaca, Morelos, Mexico.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2024 Apr 06; Vol. 10 (8), pp. e29329. Date of Electronic Publication: 2024 Apr 06 (Print Publication: 2024).
DOI:	10.1016/j.heliyon.2024.e29329
Abstrakt:	Dengue is a significant disease transmitted by Aedes mosquitoes in the tropics and subtropics worldwide. The disease is caused by four virus (DENV) serotypes and is transmitted to humans by female Aedes aegypti mosquito bites infected with the virus and vertically to their progeny. Current strategies to control dengue transmission focus on the vector. In this study, we describe an indirect Enzyme-Linked Immunosorbent Assay (ELISA), using a monoclonal antibody against the non-structural dengue virus protein 1 (NS1), to detect DENV2 in Ae. aegypti eggs. The assay detects NS1 in eggs homogenates with 87.5% sensitivity and 75.0% specificity and it is proposed as a tool for the routine entomovirological surveillance of DENV 2 in field mosquito populations. Competing Interests: The authors declare no conflicts of interest. (© 2024 The Authors.)
Databáze:	MEDLINE
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