Effect of Resveratrol on MMP-2 Expression in Scleral Fibroblasts: An In Vitro Study.

Autor:	Tang X; Department of Ophthalmology, Yongchuan Hospital, Chongqing Medical University, Chongqing, China.; Department of Ophthalmology, Anyue People's Hospital, Ziyang, Sichuan Province, China., Lv S; Department of Ophthalmology, Yongchuan Hospital, Chongqing Medical University, Chongqing, China., Liu S; Department of Ophthalmology, Yongchuan Hospital, Chongqing Medical University, Chongqing, China., Song S; Department of Ophthalmology, Yongchuan Hospital, Chongqing Medical University, Chongqing, China., Li H; Department of Ophthalmology, Yongchuan Hospital, Chongqing Medical University, Chongqing, China.
Jazyk:	angličtina
Zdroj:	Current eye research [Curr Eye Res] 2024 Sep; Vol. 49 (9), pp. 972-979. Date of Electronic Publication: 2024 Apr 28.
DOI:	10.1080/02713683.2024.2346940
Abstrakt:	Purpose: To investigate the effects of resveratrol (Res) on human fetal scleral fibroblasts (HFSFs) and its potential mechanism. Methods: HFSFs were randomly divided into the Res-treated group and the control group. Following, HFSFs were treated with or without a concentration of 10 μM Res for 48 h. To detect the expression of related genes, reverse transcription quantitative PCR (RT-qPCR) and western blotting were used. The apoptosis rate of different groups was determined using flow cytometry. Results: The mRNA expression of matrix metalloproteinase 2 (MMP-2), Collagen, Type I, Alpha 1 (COL1A1), Janus Kinase 2 (JAK2), and Signal Transducer and Activator of Transcription 3 (STAT3)" was downregulated in the Res-treatment group compared to the control group, according to RT-qPCR. Western blotting revealed that Res therapy reduced the expression of MMP-2, JAK2, P-JAK2, STAT3, P-STAT3, and Bcl-2 associated protein X (Bax) while increasing the expression of COL1A1 and B-cell lymphoma-2 (Bcl-2). Flow cytometry showed that the cell apoptosis rate was significantly lower in HFSFs treated with Res. Conclusions: In conclusion, these findings suggest that Res increases COL1A1 expression while inhibiting MMP-2 and cell apoptosis in HFSFs, possibly through modulation of the JAK2/STAT3 signaling pathway.
Databáze:	MEDLINE
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