Single-cell SERS imaging of dual cell membrane receptors expression influenced by extracellular matrix stiffness.

Autor: Liu X; Research Center for Analytical Sciences, Northeastern University, Shenyang 110819, PR China., Zhang W; Research Center for Analytical Sciences, Northeastern University, Shenyang 110819, PR China., Gu J; Research Center for Analytical Sciences, Northeastern University, Shenyang 110819, PR China., Wang J; Research Center for Analytical Sciences, Northeastern University, Shenyang 110819, PR China., Wang Y; Research Center for Analytical Sciences, Northeastern University, Shenyang 110819, PR China., Xu Z; Research Center for Analytical Sciences, Northeastern University, Shenyang 110819, PR China. Electronic address: xuzr@mail.neu.edu.cn.
Jazyk: angličtina
Zdroj: Journal of colloid and interface science [J Colloid Interface Sci] 2024 Aug 15; Vol. 668, pp. 335-342. Date of Electronic Publication: 2024 Apr 24.
DOI: 10.1016/j.jcis.2024.04.170
Abstrakt: Membrane receptors perform a diverse range of cellular functions, accounting for more than half of all drug targets. The mechanical microenvironment regulates cell behaviors and phenotype. However, conventional analysis methods of membrane receptors often ignore the effects of the extracellular matrix stiffness, failing to reveal the heterogeneity of cell membrane receptors expression. Herein, we developed an in-situ surface-enhanced Raman scattering (SERS) imaging method to visualize single-cell membrane receptors on substrates with different stiffness. Two SERS substrates, Au@4-mercaptobenzonitrile@Ag@Sgc8c and Au@4-pethynylaniline@Ag@SYL3c, were employed to specifically target protein tyrosine kinase-7 (PTK7) and epithelial cell adhesion molecule (EpCAM), respectively. The polyacrylamide (PA) gels with tunable stiffness (2.5-25 kPa) were constructed to mimic extracellular matrix. The simultaneous SERS imaging of dual membrane receptors on single cancer cells on substrates with different stiffness was achieved. Our findings reveal decreased expression of PTK7 and EpCAM on cells cultured on stiffer substrates and higher migration ability of the cells. The results elucidate the heterogeneity of membrane receptors expression of cells cultured on the substrates with different stiffness. This single-cell analysis method offers an in-situ platform for investigating the impacts of extracellular matrix stiffness on the expression of membrane receptors, providing insights into the role of cell membrane receptors in cancer metastasis.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: