Comparative evaluation of liquid-liquid extraction and nanosorbent extraction for HPLC-PDA analysis of cabazitaxel from rat plasma.

Autor: Suseela MNL; Department of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi, UP 221005, India., Mehata AK; Department of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi, UP 221005, India., Vallamkonda B; Department of Pharmaceutical Science, School of Applied Sciences and Humanities, VIGNAN'S Foundation for Science, Technology & Research, Vadlamudi 522213, India., Gokul P; Department of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi, UP 221005, India., Pradhan A; Department of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi, UP 221005, India., Pandey J; Department of Physics, Banaras Hindu University, Varanasi 221005, India., Selvin J; Department of Microbiology, Pondicherry University, Puducherry 605014, India., Sterlin Leo Hudson M; Department of Physics, Banaras Hindu University, Varanasi 221005, India., Muthu MS; Department of Pharmaceutical Engineering and Technology, Indian Institute of Technology (BHU), Varanasi, UP 221005, India. Electronic address: msmuthu.phe@itbhu.ac.in.
Jazyk: angličtina
Zdroj: Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2024 Aug 01; Vol. 245, pp. 116149. Date of Electronic Publication: 2024 Apr 16.
DOI: 10.1016/j.jpba.2024.116149
Abstrakt: A precise, sensitive, accurate, and validated reverse-phase high-performance liquid chromatography (RP-HPLC) method with a bioanalytical approach was utilized to analyze Cabazitaxel (CBZ) in rat plasma. Comparative research on extraction recoveries was performed between traditional liquid-liquid extraction (LLE) and synthesized graphene oxide (GO) based magnetic solid phase extraction (GO@MSPE). The superparamagnetic hybrid nanosorbent was synthesized using the combination of iron oxide and GO and subsequently applied for extraction and bioanalytical quantification of CBZ from plasma by (HPLC-PDA) analysis. Fourier- transform infrared spectroscopy (FT-IR), particle size, scanning electron microscopy (SEM), and x-ray diffraction (XRD) analysis were employed in the characterization of synthesized GO@MSPE nanosorbent. The investigation was accomplished using a shim pack C18 column (150 mm×4.6 mm, 5 µm) with a binary gradient mobile phase consisting of formic acid: acetonitrile: water (0.1:75:25, v/v/v) at a 0.8 mL/min flow rate, and a λ max of 229 nm. The limits of detection (LOD) and quantitation (LOQ) have been determined to be 50 and 100 ng/mL for both LLE and SPE techniques. The linearity range of the approach encompassed from 100 to 5000 ng/mL and was found to be linear (coefficient of determination > 0.99) for CBZ. The proposed method showed extraction recovery of 76.8-88.4% for the synthesized GO@MSPE and 69.3-77.4% for LLE, suggesting that the proposed bioanalytical approach was robust and qualified for all validation parameters within the acceptable criteria. Furthermore, the developed hybrid GO@MSPE nanosorbent with the help of the proposed RP-HPLC method, showed a significant potential for the extraction of CBZ in bioanalysis.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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