Detection of metallo-beta-lactamase-producing genes bla SPM and bla NDM in Pseudomonas aeruginosa isolated from wastewater in Southern Brazil.

Autor: Monteagudo de Barros V; Molecular Microbiology Laboratory, Institute of Health Sciences, Feevale University, Red Building 2nd Floor, Room 205, RS 239, No. 2755, Vila Nova, Novo Hamburgo, RS 93352-000, Brazil E-mail: vini.monteagudo@gmail.com., da Rocha BS; Molecular Microbiology Laboratory, Institute of Health Sciences, Feevale University, Red Building 2nd Floor, Room 205, RS 239, No. 2755, Vila Nova, Novo Hamburgo, RS 93352-000, Brazil., Rhoden J; Molecular Microbiology Laboratory, Institute of Health Sciences, Feevale University, Red Building 2nd Floor, Room 205, RS 239, No. 2755, Vila Nova, Novo Hamburgo, RS 93352-000, Brazil., Stein JF; Molecular Microbiology Laboratory, Institute of Health Sciences, Feevale University, Red Building 2nd Floor, Room 205, RS 239, No. 2755, Vila Nova, Novo Hamburgo, RS 93352-000, Brazil., Picoli SU; Integrated Health Specialties Center - CIES Feevale, Institute of Health Sciences, Feevale University, R. Rubem Berta, 200 - Vila Nova, Novo Hamburgo, RS 93525-080, Brazil., Rigotto C; Molecular Microbiology Laboratory, Institute of Health Sciences, Feevale University, Red Building 2nd Floor, Room 205, RS 239, No. 2755, Vila Nova, Novo Hamburgo, RS 93352-000, Brazil.
Jazyk: angličtina
Zdroj: Journal of water and health [J Water Health] 2024 Apr; Vol. 22 (4), pp. 689-700. Date of Electronic Publication: 2024 Mar 14.
DOI: 10.2166/wh.2024.305
Abstrakt: Pseudomonas aeruginosa is commonly associated with the ability to acquire antimicrobial resistance. The surveillance of resistance genes in various environmental matrices has gained prominence in recent years, being seen as a potential threat to public health. The objective of this study was to investigate genes encoding metallo-beta-lactamases (MBLs), which confer resistance to carbapenems, in wastewater. Fifteen isolates of P. aeruginosa were collected for five months from samples obtained from a municipal wastewater treatment plant in Rio Grande do Sul. These isolates were subjected to disk diffusion testing using 10 different antimicrobials. Phenotypic enzymatic tests for MBLs were conducted, and positive isolates underwent DNA extraction and gene detection using the polymerase chain reaction. The resistance rate to ceftazidime was 100%, cefepime 73.3%, piperacillin-tazobactam 66.67%, imipenem 53.30%, levofloxacin 46.67%, tobramycin 40%, and ciprofloxacin and amikacin 13.33%. Both meropenem and aztreonam resistances were rare accounting for 6.60% of the tested isolates. Among these isolates, 20% were classified as multidrug-resistant and were found to carry the bla NDM and bla SPM genes. The results suggest that evaluating resistance genes in bacteria from urban raw sewage can provide data that assist in surveillance, as this environment can stimulate increased bacterial resistance.
Competing Interests: The authors declare there is no conflict.
(© 2024 The Authors This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC BY 4.0), which permits copying, adaptation and redistribution, provided the original work is properly cited (http://creativecommons.org/licenses/by/4.0/).)
Databáze: MEDLINE
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