Lifecycle of a predatory bacterium vampirizing its prey through the cell envelope and S-layer.

Autor: Santin YG; de Duve Institute, UCLouvain, 75 avenue Hippocrate, 1200, Brussels, Belgium., Sogues A; Structural and Molecular Microbiology, Structural Biology Research Center, VIB, Pleinlaan 2, 1050, Brussels, Belgium.; Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium., Bourigault Y; de Duve Institute, UCLouvain, 75 avenue Hippocrate, 1200, Brussels, Belgium., Remaut HK; Structural and Molecular Microbiology, Structural Biology Research Center, VIB, Pleinlaan 2, 1050, Brussels, Belgium.; Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium., Laloux G; de Duve Institute, UCLouvain, 75 avenue Hippocrate, 1200, Brussels, Belgium. geraldine.laloux@uclouvain.be.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2024 Apr 27; Vol. 15 (1), pp. 3590. Date of Electronic Publication: 2024 Apr 27.
DOI: 10.1038/s41467-024-48042-5
Abstrakt: Predatory bacteria feed upon other bacteria in various environments. Bdellovibrio exovorus is an obligate epibiotic predator that attaches on the prey cell surface, where it grows and proliferates. Although the mechanisms allowing feeding through the prey cell envelope are unknown, it has been proposed that the prey's proteinaceous S-layer may act as a defensive structure against predation. Here, we use time-lapse and cryo-electron microscopy to image the lifecycle of B. exovorus feeding on Caulobacter crescentus. We show that B. exovorus proliferates by non-binary division, primarily generating three daughter cells. Moreover, the predator feeds on C. crescentus regardless of the presence of an S-layer, challenging its assumed protective role against predators. Finally, we show that apparently secure junctions are established between prey and predator outer membranes.
(© 2024. The Author(s).)
Databáze: MEDLINE
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