Development of a polarity-sensitive ratiometric fluorescent probe based on the intramolecular reaction of spiro-oxazolidine and its applications for in situ visualizing the fluctuations of polarity during ER stress.

Autor: Jiang Z; School of Chemistry and Chemical Engineering, University of Jinan, Jinan, Shandong 250022, PR China., Dai X; School of Chemistry and Chemical Engineering, University of Jinan, Jinan, Shandong 250022, PR China., Zhou L; School of Chemistry and Chemical Engineering, University of Jinan, Jinan, Shandong 250022, PR China., Yang Z; School of Chemistry and Chemical Engineering, University of Jinan, Jinan, Shandong 250022, PR China., Yu F; School of Chemistry and Chemical Engineering, University of Jinan, Jinan, Shandong 250022, PR China. Electronic address: chm_yufq@ujn.edu.cn., Kong X; School of Chemistry and Chemical Engineering, University of Jinan, Jinan, Shandong 250022, PR China. Electronic address: ifp_kongxq@ujn.edu.cn.
Jazyk: angličtina
Zdroj: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2024 Aug 05; Vol. 316, pp. 124337. Date of Electronic Publication: 2024 Apr 23.
DOI: 10.1016/j.saa.2024.124337
Abstrakt: Polarity is a vital element in endoplasmic reticulum (ER) microenvironment, and its variation is closely related to many physiological and pathological activities of ER, so it is necessary to trace fluctuations of polarity in ER. However, most of fluorescent probes for detecting polarity dependent on the changes of single emission, which could be affected by many factors and cause false signals. Ratiometric fluorescent probe with "built-in calibration" can effectively avoid detection errors. Here, we have designed a ratiometric fluorescent probe HM for monitoring the ER polarity based on the intramolecular reaction of spiro-oxazolidine. It forms ring open/closed isomers driven by polarity to afford ratiometric sensing. Probe HM have manifested its ratiometric responses to polarity in spectroscopic results, which could offer much more precise information for the changes of polarity in living cells with the internal built-in correction. It also showed large emission shift ( 133 nm), high selectivity and photo-stability. In biological imaging, HM could selectively accumulate in ER with high photo-stability. Importantly, HM has ability for in situ tracing the changes of ER polarity with ratiometric behavior during the ER stress process with the stimulation of tunicamycin, dithiothreitol and hypoxia, suggesting that HM is an effective molecule tool for monitoring the variations of ER polarity.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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