Genome and secretome insights: unravelling the lignocellulolytic potential of Myceliophthora verrucosa for enhanced hydrolysis of lignocellulosic biomass.

Autor: Sharma G; Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India., Kaur B; Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India., Singh V; Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India., Raheja Y; Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India., Falco MD; Center for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montreal, QC, H4B 1R6, Canada., Tsang A; Center for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montreal, QC, H4B 1R6, Canada., Chadha BS; Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India. chadhabs@yahoo.com.
Jazyk: angličtina
Zdroj: Archives of microbiology [Arch Microbiol] 2024 Apr 27; Vol. 206 (5), pp. 236. Date of Electronic Publication: 2024 Apr 27.
DOI: 10.1007/s00203-024-03974-w
Abstrakt: Lignocellulolytic enzymes from a novel Myceliophthora verrucosa (5DR) strain was found to potentiate the efficacy of benchmark cellulase during saccharification of acid/alkali treated bagasse by ~ 2.24 fold, indicating it to be an important source of auxiliary enzymes. The De-novo sequencing and analysis of M. verrucosa genome (31.7 Mb) revealed to encode for 7989 putative genes, representing a wide array of CAZymes (366) with a high proportions of auxiliary activity (AA) genes (76). The LC/MS QTOF based secretome analysis of M. verrucosa showed high abundance of glycosyl hydrolases and AA proteins with cellobiose dehydrogenase (CDH) (AA8), being the most prominent auxiliary protein. A gene coding for lytic polysaccharide monooxygenase (LPMO) was expressed in Pichia pastoris and CDH produced by M. verrucosa culture on rice straw based solidified medium were purified and characterized. The mass spectrometry of LPMO catalyzed hydrolytic products of avicel showed the release of both C1/C4 oxidized products, indicating it to be type-3. The lignocellulolytic cocktail comprising of in-house cellulase produced by Aspergillus allahabadii strain spiked with LPMO & CDH exhibited enhanced and better hydrolysis of mild alkali deacetylated (MAD) and unwashed acid pretreated rice straw slurry (UWAP), when compared to Cellic CTec3 at high substrate loading rate.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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