The cuticle proteome of a planktonic crustacean.

Autor: Otte KA; Institute of Cell and Systems Biology of Animals, University of Hamburg, Hamburg, Germany., Fredericksen M; Department of Environmental Sciences, Zoology, University of Basel, Basel, Switzerland., Fields P; Department of Environmental Sciences, Zoology, University of Basel, Basel, Switzerland., Fröhlich T; Laboratory for Functional Genome Analysis, Gene Center, LMU Munich, Munich, Germany., Laforsch C; Animal Ecology 1, University of Bayreuth, Bayreuth, Germany., Ebert D; Department of Environmental Sciences, Zoology, University of Basel, Basel, Switzerland.
Jazyk: angličtina
Zdroj: Proteomics [Proteomics] 2024 Jul; Vol. 24 (14), pp. e2300292. Date of Electronic Publication: 2024 Apr 27.
DOI: 10.1002/pmic.202300292
Abstrakt: The cuticles of arthropods provide an interface between the organism and its environment. Thus, the cuticle's structure influences how the organism responds to and interacts with its surroundings. Here, we used label-free quantification proteomics to provide a proteome of the moulted cuticle of the aquatic crustacean Daphnia magna, which has long been a prominent subject of studies on ecology, evolution, and developmental biology. We detected a total of 278 high-confidence proteins. Using protein sequence domain and functional enrichment analyses, we identified chitin-binding structural proteins and chitin-modifying enzymes as the most abundant protein groups in the cuticle proteome. Structural cuticular protein families showed a similar distribution to those found in other arthropods and indicated proteins responsible for the soft and flexible structure of the Daphnia cuticle. Finally, cuticle protein genes were also clustered as tandem gene arrays in the D. magna genome. The cuticle proteome presented here will be a valuable resource to the Daphnia research community, informing genome annotations and investigations on diverse topics such as the genetic basis of interactions with predators and parasites.
(© 2024 The Authors. PROTEOMICS published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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