Pandemic Risk Assessment for Swine Influenza A Virus in Comparative In Vitro and In Vivo Models.

Autor:	Padykula I; Center for Vaccines and Immunology, University of Georgia, Athens, GA 30602, USA.; Department of Infectious Diseases, University of Georgia, Athens, GA 30602, USA.; Emory-UGA Centers of Excellence for Influenza Research and Surveillance (CEIRS), Athens, GA 30602, USA., Damodaran L; Department of Infectious Diseases, University of Georgia, Athens, GA 30602, USA.; Emory-UGA Centers of Excellence for Influenza Research and Surveillance (CEIRS), Athens, GA 30602, USA., Young KT; Department of Pathology, University of Georgia, Athens, GA 30602, USA., Krunkosky M; Center for Vaccines and Immunology, University of Georgia, Athens, GA 30602, USA.; Department of Infectious Diseases, University of Georgia, Athens, GA 30602, USA.; Emory-UGA Centers of Excellence for Influenza Research and Surveillance (CEIRS), Athens, GA 30602, USA., Griffin EF; Center for Vaccines and Immunology, University of Georgia, Athens, GA 30602, USA.; Department of Infectious Diseases, University of Georgia, Athens, GA 30602, USA.; Emory-UGA Centers of Excellence for Influenza Research and Surveillance (CEIRS), Athens, GA 30602, USA., North JF; Emory-UGA Centers of Excellence for Influenza Research and Surveillance (CEIRS), Athens, GA 30602, USA.; Department of Pathobiology, Auburn University, Auburn, AL 36849, USA., Neasham PJ; Emory-UGA Centers of Excellence for Influenza Research and Surveillance (CEIRS), Athens, GA 30602, USA.; Department of Pathobiology, Auburn University, Auburn, AL 36849, USA., Pliasas VC; Emory-UGA Centers of Excellence for Influenza Research and Surveillance (CEIRS), Athens, GA 30602, USA.; Department of Pathobiology, Auburn University, Auburn, AL 36849, USA., Siepker CL; Department of Pathology, University of Georgia, Athens, GA 30602, USA., Stanton JB; Department of Pathology, University of Georgia, Athens, GA 30602, USA., Howerth EW; Department of Pathology, University of Georgia, Athens, GA 30602, USA., Bahl J; Department of Infectious Diseases, University of Georgia, Athens, GA 30602, USA., Kyriakis CS; Emory-UGA Centers of Excellence for Influenza Research and Surveillance (CEIRS), Athens, GA 30602, USA.; Department of Pathobiology, Auburn University, Auburn, AL 36849, USA., Tompkins SM; Center for Vaccines and Immunology, University of Georgia, Athens, GA 30602, USA.; Department of Infectious Diseases, University of Georgia, Athens, GA 30602, USA.; Emory-UGA Centers of Excellence for Influenza Research and Surveillance (CEIRS), Athens, GA 30602, USA.
Jazyk:	angličtina
Zdroj:	Viruses [Viruses] 2024 Mar 31; Vol. 16 (4). Date of Electronic Publication: 2024 Mar 31.
DOI:	10.3390/v16040548
Abstrakt:	Swine influenza A viruses pose a public health concern as novel and circulating strains occasionally spill over into human hosts, with the potential to cause disease. Crucial to preempting these events is the use of a threat assessment framework for human populations. However, established guidelines do not specify which animal models or in vitro substrates should be used. We completed an assessment of a contemporary swine influenza isolate, A/swine/GA/A27480/2019 (H1N2), using animal models and human cell substrates. Infection studies in vivo revealed high replicative ability and a pathogenic phenotype in the swine host, with replication corresponding to a complementary study performed in swine primary respiratory epithelial cells. However, replication was limited in human primary cell substrates. This contrasted with our findings in the Calu-3 cell line, which demonstrated a replication profile on par with the 2009 pandemic H1N1 virus. These data suggest that the selection of models is important for meaningful risk assessment.
Databáze:	MEDLINE
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