Comparative Performance in the Detection of Four Coronavirus Genera from Human, Animal, and Environmental Specimens.

Autor: Wacharapluesadee S; Thai Red Cross Emerging Infectious Diseases Clinical Center, King Chulalongkorn Memorial Hospital, Bangkok 10330, Thailand.; Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand., Thippamom N; Thai Red Cross Emerging Infectious Diseases Clinical Center, King Chulalongkorn Memorial Hospital, Bangkok 10330, Thailand., Hirunpatrawong P; Thai Red Cross Emerging Infectious Diseases Clinical Center, King Chulalongkorn Memorial Hospital, Bangkok 10330, Thailand.; Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand., Rattanatumhi K; Thai Red Cross Emerging Infectious Diseases Clinical Center, King Chulalongkorn Memorial Hospital, Bangkok 10330, Thailand., Sterling SL; Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.; Henry M. Jackson Foundation, Bethesda, MD 20817, USA., Khunnawutmanotham W; Thai Red Cross Emerging Infectious Diseases Clinical Center, King Chulalongkorn Memorial Hospital, Bangkok 10330, Thailand., Noradechanon K; Department of National Parks, Wildlife and Plant Conservation, Ministry of Natural Resources and Environment, Bangkok 10900, Thailand., Maneeorn P; Department of National Parks, Wildlife and Plant Conservation, Ministry of Natural Resources and Environment, Bangkok 10900, Thailand., Buathong R; Department of Disease Control, Ministry of Public Health, Muang, Nonthaburi 11000, Thailand., Paitoonpong L; Thai Red Cross Emerging Infectious Diseases Clinical Center, King Chulalongkorn Memorial Hospital, Bangkok 10330, Thailand.; Division of Infectious Diseases, Department of Medicine, Faculty of Medicine Chulalongkorn University, Bangkok 10330, Thailand., Putcharoen O; Thai Red Cross Emerging Infectious Diseases Clinical Center, King Chulalongkorn Memorial Hospital, Bangkok 10330, Thailand.; Division of Infectious Diseases, Department of Medicine, Faculty of Medicine Chulalongkorn University, Bangkok 10330, Thailand.
Jazyk: angličtina
Zdroj: Viruses [Viruses] 2024 Mar 29; Vol. 16 (4). Date of Electronic Publication: 2024 Mar 29.
DOI: 10.3390/v16040534
Abstrakt: Emerging coronaviruses (CoVs) are understood to cause critical human and domestic animal diseases; the spillover from wildlife reservoirs can result in mild and severe respiratory illness in humans and domestic animals and can spread more readily in these naïve hosts. A low-cost CoV molecular method that can detect a variety of CoVs from humans, animals, and environmental specimens is an initial step to ensure the early identification of known and new viruses. We examine a collection of 50 human, 46 wastewater, 28 bat, and 17 avian archived specimens using 3 published pan-CoV PCR assays called Q-, W-, and X-CoV PCR, to compare the performance of each assay against four CoV genera. X-CoV PCR can detect all four CoV genera, but Q- and W-CoV PCR failed to detect δ-CoV. In total, 21 (42.0%), 9 (18.0%), and 21 (42.0%) of 50 human specimens and 30 (65.22%), 6 (13.04%), and 27 (58.70%) of 46 wastewater specimens were detected using Q-, W-, and X-CoV PCR assays, respectively. The X-CoV PCR assay has a comparable sensitivity to Q-CoV PCR in bat CoV detection. Combining Q- and X-CoV PCR assays can increase sensitivity and avoid false negative results in the early detection of novel CoVs.
Competing Interests: The authors declare no conflicts of interest.
Databáze: MEDLINE
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