| Autor: |
Ciocan Moraru A; Faculty of Medicine, Department of Pharmacology, University of Medicine and Pharmacy 'Grigore T. Popa', 700115 Iaşi, Romania.; Clinical Hospital of Pulmonary Diseases, 400012 Iaşi, Romania., Ciubotariu D; Faculty of Medicine, Department of Pharmacology, University of Medicine and Pharmacy 'Grigore T. Popa', 700115 Iaşi, Romania.; 'St. Maria' Clinical Hospital for Children, 700309 Iaşi, Romania., Ghiciuc CM; Faculty of Medicine, Department of Pharmacology, University of Medicine and Pharmacy 'Grigore T. Popa', 700115 Iaşi, Romania.; 'St. Maria' Clinical Hospital for Children, 700309 Iaşi, Romania., Hurmuzache ME; Faculty of Medicine, Department of Pharmacology, University of Medicine and Pharmacy 'Grigore T. Popa', 700115 Iaşi, Romania.; 'St. Parascheva' Hospital for Infectious Diseases, 700116 Iaşi, Romania., Lupușoru CE; Faculty of Medicine, Department of Pharmacology, University of Medicine and Pharmacy 'Grigore T. Popa', 700115 Iaşi, Romania., Crișan-Dabija R; Faculty of Medicine, Department of Pharmacology, University of Medicine and Pharmacy 'Grigore T. Popa', 700115 Iaşi, Romania.; Clinical Hospital of Pulmonary Diseases, 400012 Iaşi, Romania. |
| Jazyk: |
angličtina |
| Zdroj: |
Medicina (Kaunas, Lithuania) [Medicina (Kaunas)] 2024 Apr 08; Vol. 60 (4). Date of Electronic Publication: 2024 Apr 08. |
| DOI: |
10.3390/medicina60040611 |
| Abstrakt: |
Background and Objectives : The purpose of this study was to investigate the influence induced by magnesium chloride (MgCl 2 ) and zinc gluconate (ZnG) supplementation on liver and kidney injuries experimentally induced with acetaminophen (AAPh) and potentiated by a ciprofloxacin addition in rats. Material and Methods : The experiment was performed on five animal groups: group 1-control, treated for 6 weeks with normal saline, 1 mL/kg; group 2-AAPh, treated for 6 weeks with AAPh, 100 mg/kg/day; group 3-AAPh + C, treated for 6 weeks with AAPh 100 mg/kg/day and ciprofloxacin 50 mg/kg/day, only in the last 14 days of the experiment; group 4-AAPh + C + Mg, with the same treatment as group 3, but in the last 14 days, MgCl 2 10 mg/ kg/day was added; and group 5-AAPh + C + Zn, with the same treatment as group 3, but in the last 14 days, zinc gluconate (ZnG), 10 mg/kg/day was added. All administrations were performed by oral gavage. At the end of the experiment, the animals were sacrificed and blood samples were collected for biochemistry examinations. Results : Treatment with AAPh for 6 weeks determined an alteration of the liver function (increases in alanine aminotransferase, aspartate aminotransferase, lactic dehydrogenase, and gamma-glutamyl transferase) and of renal function (increases in serum urea and creatinine) ( p < 0.001 group 2 vs. group 1 for all mentioned parameters). Furthermore, the antioxidant defense capacity was impaired in group 2 vs. group 1 (superoxide dismutase and glutathione peroxidase activity decreased in group 2 vs. group 1, at 0.001 < p < 0.01 and 0.01 < p < 0.05, respectively). The addition of ciprofloxacin, 50 mg/kg/day during the last 14 days, resulted in further increases in alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, urea, and creatinine (0.01 < p < 0.05, group 3 vs. group 2). MgCl 2 provided a slight protection against the increase in liver enzymes, and a more pronounced protection against the increase in serum urea and creatinine (0.001 < p < 0.01 group 4 vs. group 3). MgCl 2 provided a slight protection against the decrease in superoxide dismutase (0.01 < p < 0.05 group 4 vs. group 3), but not against decrease of glutathione peroxidase. The improvement of mentioned parameters could also be seen in the case of ZnG, to a higher extent, especially in the case of alanine aminotransferase and lactic dehydrogenase (0.01 < p < 0.05 group 5 vs. group 4). Conclusions : This study presents further proof for the beneficial effect of magnesium and zinc salts against toxicity induced by different agents, including antibacterials added to the analgesic and antipyretic acetaminophen; the protection is proven on the liver and kidney's function, and the antioxidant profile improvement has a key role, especially in the case of zinc gluconate. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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