Effect of a 12-Week Walking Program Monitored by Global Physical Capacity Score (GPCS) on Circulating Cell-Free mtDNA and DNase Activity in Patients with Irritable Bowel Syndrome.

Autor: Chimienti G; Department of Biosciences, Biotechnologies and Environment, University of Bari Aldo Moro, 70125 Bari, Italy., Russo F; Functional Gastrointestinal Disorders Research Group, National Institute of Gastroenterology IRCCS 'Saverio de Bellis', 70013 Castellana Grotte, Italy., Bianco A; Laboratory of Movement and Wellness, National Institute of Gastroenterology IRCCS 'Saverio de Bellis', 70013 Castellana Grotte, Italy., Maqoud F; Functional Gastrointestinal Disorders Research Group, National Institute of Gastroenterology IRCCS 'Saverio de Bellis', 70013 Castellana Grotte, Italy., De Virgilio C; Department of Biosciences, Biotechnologies and Environment, University of Bari Aldo Moro, 70125 Bari, Italy., Galeano G; Functional Gastrointestinal Disorders Research Group, National Institute of Gastroenterology IRCCS 'Saverio de Bellis', 70013 Castellana Grotte, Italy., Orlando A; Functional Gastrointestinal Disorders Research Group, National Institute of Gastroenterology IRCCS 'Saverio de Bellis', 70013 Castellana Grotte, Italy., Riezzo G; Functional Gastrointestinal Disorders Research Group, National Institute of Gastroenterology IRCCS 'Saverio de Bellis', 70013 Castellana Grotte, Italy., D'Attoma B; Functional Gastrointestinal Disorders Research Group, National Institute of Gastroenterology IRCCS 'Saverio de Bellis', 70013 Castellana Grotte, Italy., Ignazzi A; Functional Gastrointestinal Disorders Research Group, National Institute of Gastroenterology IRCCS 'Saverio de Bellis', 70013 Castellana Grotte, Italy., Linsalata M; Functional Gastrointestinal Disorders Research Group, National Institute of Gastroenterology IRCCS 'Saverio de Bellis', 70013 Castellana Grotte, Italy., Prospero L; Functional Gastrointestinal Disorders Research Group, National Institute of Gastroenterology IRCCS 'Saverio de Bellis', 70013 Castellana Grotte, Italy., Franco I; Laboratory of Movement and Wellness, National Institute of Gastroenterology IRCCS 'Saverio de Bellis', 70013 Castellana Grotte, Italy., Bagnato CB; Laboratory of Movement and Wellness, National Institute of Gastroenterology IRCCS 'Saverio de Bellis', 70013 Castellana Grotte, Italy., Curci R; Laboratory of Movement and Wellness, National Institute of Gastroenterology IRCCS 'Saverio de Bellis', 70013 Castellana Grotte, Italy., Coletta S; Core Facility Biobank, National Institute of Gastroenterology IRCCS 'Saverio de Bellis', 70013 Castellana Grotte, Italy.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2024 Apr 12; Vol. 25 (8). Date of Electronic Publication: 2024 Apr 12.
DOI: 10.3390/ijms25084293
Abstrakt: Irritable bowel syndrome (IBS) involves low-grade mucosal inflammation. Among the various approaches capable of managing the symptoms, physical activity is still under investigation. Despite its benefits, it promotes oxidative stress and inflammation. Mitochondria impacts gut disorders by releasing damage-associated molecular patterns, such as cell-free mtDNA (cf-mtDNA), which support inflammation. This study evaluated the effects of a 12-week walking program on the cf-mtDNA and DNase in 26 IBS and 17 non-IBS subjects. Pro- and anti-inflammatory cytokines were evaluated by ELISA. Digital droplet PCR was used to quantify cf-mtDNA; DNase activity was assessed using a single radial enzyme diffusion assay. PCR-RFLP was used to genotype DNASE1 rs1053874 SNP. Significantly lower IL-10 levels were found in IBS than in non-IBS individuals. Exercise reduced cf-mtDNA in non-IBS subjects but not in IBS patients. DNase activity did not correlate with the cf-mtDNA levels in IBS patients post-exercise, indicating imbalanced cf-mtDNA clearance. Different rs1053874 SNP frequencies were not found between groups. The study confirms the positive effects of regular moderate-intensity physical activity in healthy subjects and its role in cf-mtDNA release and clearance. Walking alone might not sufficiently reduce subclinical inflammation in IBS, based on imbalanced pro- and anti-inflammatory molecules. Prolonged programs are necessary to investigate their effects on inflammatory markers in IBS.
Databáze: MEDLINE
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