Mitochondrial DNA and Inflammation Are Associated with Cerebral Vessel Remodeling and Early Diabetic Kidney Disease in Patients with Type 2 Diabetes Mellitus.

Autor: Petrica L; Department of Internal Medicine II, Division of Nephrology, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; Centre for Molecular Research in Nephrology and Vascular Disease, Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; Centre for Cognitive Research in Neuropsychiatric Pathology (Neuropsy-Cog), Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; Center for Translational Research and Systems Medicine, Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; County Emergency Hospital Timisoara, 300723 Timisoara, Romania., Gadalean F; Department of Internal Medicine II, Division of Nephrology, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; Centre for Molecular Research in Nephrology and Vascular Disease, Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; County Emergency Hospital Timisoara, 300723 Timisoara, Romania., Muntean DM; Centre for Molecular Research in Nephrology and Vascular Disease, Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; Center for Translational Research and Systems Medicine, Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; Department of Functional Sciences III, Division of Pathophysiology, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania., Jianu DC; Centre for Molecular Research in Nephrology and Vascular Disease, Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; Centre for Cognitive Research in Neuropsychiatric Pathology (Neuropsy-Cog), Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; County Emergency Hospital Timisoara, 300723 Timisoara, Romania.; Department of Neurosciences VIII, Division of Neurology I, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania., Vlad D; Centre for Molecular Research in Nephrology and Vascular Disease, Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; County Emergency Hospital Timisoara, 300723 Timisoara, Romania.; Department of Biochemistry and Pharmacology IV, Division of Pharmacology, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania., Dumitrascu V; Centre for Molecular Research in Nephrology and Vascular Disease, Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; County Emergency Hospital Timisoara, 300723 Timisoara, Romania.; Department of Biochemistry and Pharmacology IV, Division of Pharmacology, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania., Bob F; Department of Internal Medicine II, Division of Nephrology, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; Centre for Molecular Research in Nephrology and Vascular Disease, Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; County Emergency Hospital Timisoara, 300723 Timisoara, Romania., Milas O; Department of Internal Medicine II, Division of Nephrology, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; Centre for Molecular Research in Nephrology and Vascular Disease, Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; County Emergency Hospital Timisoara, 300723 Timisoara, Romania., Suteanu-Simulescu A; Department of Internal Medicine II, Division of Nephrology, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; Centre for Molecular Research in Nephrology and Vascular Disease, Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; County Emergency Hospital Timisoara, 300723 Timisoara, Romania., Glavan M; Department of Internal Medicine II, Division of Nephrology, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; Centre for Molecular Research in Nephrology and Vascular Disease, Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; County Emergency Hospital Timisoara, 300723 Timisoara, Romania., Ursoniu S; Centre for Molecular Research in Nephrology and Vascular Disease, Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; Centre for Cognitive Research in Neuropsychiatric Pathology (Neuropsy-Cog), Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; Center for Translational Research and Systems Medicine, Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; County Emergency Hospital Timisoara, 300723 Timisoara, Romania.; Department of Functional Sciences III, Division of Public Health and History of Medicine, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania., Balint L; Department of Internal Medicine II, Division of Nephrology, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; Centre for Molecular Research in Nephrology and Vascular Disease, Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; County Emergency Hospital Timisoara, 300723 Timisoara, Romania., Mogos-Stefan M; Department of Internal Medicine II, Division of Nephrology, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; Centre for Molecular Research in Nephrology and Vascular Disease, Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; County Emergency Hospital Timisoara, 300723 Timisoara, Romania., Ienciu S; Department of Internal Medicine II, Division of Nephrology, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; Centre for Molecular Research in Nephrology and Vascular Disease, Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; County Emergency Hospital Timisoara, 300723 Timisoara, Romania., Cretu OM; Department of Surgery I, Division of Surgical Semiology I, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; Emergency Clinical Municipal Hospital Timisoara, 300041 Timisoara, Romania., Popescu R; Centre for Molecular Research in Nephrology and Vascular Disease, Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; County Emergency Hospital Timisoara, 300723 Timisoara, Romania.; Department of Microscopic Morphology II, Division of Cell and Molecular Biology II, 'Victor Babes', University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania., Gluhovschi C; Department of Internal Medicine II, Division of Nephrology, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; Centre for Molecular Research in Nephrology and Vascular Disease, Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania., Iancu L; Department of Internal Medicine II, Division of Nephrology, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; Centre for Molecular Research in Nephrology and Vascular Disease, Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; County Emergency Hospital Timisoara, 300723 Timisoara, Romania., Vlad A; Centre for Molecular Research in Nephrology and Vascular Disease, Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.; County Emergency Hospital Timisoara, 300723 Timisoara, Romania.; Department of Internal Medicine II, Division of Diabetes, Nutrition, and Metabolic Diseases, 'Victor Babes' University of Medicine and Pharmacy, No. 2, Eftimie Murgu Sq., 300041 Timisoara, Romania.
Jazyk: angličtina
Zdroj: Biomolecules [Biomolecules] 2024 Apr 19; Vol. 14 (4). Date of Electronic Publication: 2024 Apr 19.
DOI: 10.3390/biom14040499
Abstrakt: Cerebrovascular disease accounts for major neurologic disabilities in patients with type 2 diabetes mellitus (DM). A potential association of mitochondrial DNA ( mtDNA ) and inflammation with cerebral vessel remodeling in patients with type 2 DM was evaluated. A cohort of 150 patients and 30 healthy controls were assessed concerning urinary albumin/creatinine ratio (UACR), synaptopodin, podocalyxin, kidney injury molecule-1 (KIM-1), N-acetyl-β-(D)-glucosaminidase (NAG), interleukins IL-17A, IL-18, IL-10, tumor necrosis factor-alpha (TNFα), intercellular adhesion molecule-1 (ICAM-1). MtDNA-CN and nuclear DNA ( nDNA ) were quantified in peripheral blood and urine by qRT-PCR. Cytochrome b ( CYTB ) gene, subunit 2 of NADH dehydrogenase ( ND2 ), and beta 2 microglobulin nuclear gene ( B2M ) were assessed by TaqMan assays. mtDNA-CN was defined as the ratio of the number of mtDNA/nDNA copies, through analysis of the CYTB/B2M and ND2/B2M ratio; cerebral Doppler ultrasound: intima-media thickness (IMT)-the common carotid arteries (CCAs), the pulsatility index (PI) and resistivity index (RI)- the internal carotid arteries (ICAs) and middle cerebral arteries (MCAs), the breath-holding index (BHI). The results showed direct correlations of CCAs-IMT, PI-ICAs, PI-MCAs, RI-ICAs, RI-MCAs with urinary mtDNA , IL-17A, IL-18, TNFα, ICAM-1, UACR, synaptopodin, podocalyxin, KIM-1, NAG, and indirect correlations with serum mtDNA , IL-10. BHI correlated directly with serum IL-10, and serum mtDNA , and negatively with serum IL-17A, serum ICAM-1, and NAG. In neurologically asymptomatic patients with type 2 DM cerebrovascular remodeling and impaired cerebrovascular reactivity may be associated with mtDNA variations and inflammation from the early stages of diabetic kidney disease.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje