| Autor: |
Schiano-Lomoriello D; I.R.C.C.S.-G.B. Bietti Foundation, 00198 Rome, Italy., Abicca I; I.R.C.C.S.-G.B. Bietti Foundation, 00198 Rome, Italy., Contento L; I.R.C.C.S.-G.B. Bietti Foundation, 00198 Rome, Italy., Gabrielli F; Biolab SRL, Laboratorio di Genetica e Genomica Molecolare, Largo degli Aranci, 9, 63100 Ascoli Piceno, Italy., Alfonsi C; Biolab SRL, Laboratorio di Genetica e Genomica Molecolare, Largo degli Aranci, 9, 63100 Ascoli Piceno, Italy., Di Pietro F; Biolab SRL, Laboratorio di Genetica e Genomica Molecolare, Largo degli Aranci, 9, 63100 Ascoli Piceno, Italy., Papa FT; Biolab SRL, Laboratorio di Genetica e Genomica Molecolare, Largo degli Aranci, 9, 63100 Ascoli Piceno, Italy., Ballesteros-Sánchez A; Department of Physics of Condensed Matter, Optics Area, University of Seville, 41004 Seville, Spain.; Department of Ophthalmology, Clínica Novovisión, 30008 Murcia, Spain., Sánchez-González JM; Department of Physics of Condensed Matter, Optics Area, University of Seville, 41004 Seville, Spain., Rocha-De-Lossada C; Regional University Hospital of Malaga, Hospital Civil Square, 29009 Malaga, Spain.; Department of Surgery, Ophthalmology Area, University of Seville, 41009 Seville, Spain., Mazzotta C; Siena Crosslinking Center, 53100 Siena, Italy., Giannaccare G; Eye Clinic, Department of Surgical Sciences, University of Cagliari, 09121 Cagliari, Italy., Bonzano C; DiNOGMI, University of Genoa and IRCCS San Martino Polyclinic Hospital, 16132 Genoa, Italy., Borroni D; Department of Ophthalmology, Riga Stradins University, LV-1007 Riga, Latvia.; Eyemetagenomics Ltd., 71-75, Shelton Street, Covent Garden, London WC2H 9JQ, UK. |
| Abstrakt: |
Purpose : To characterize microbial keratitis diversity utilizing species richness and Shannon Diversity Index. Methods : Corneal impression membrane was used to collect samples. All swabs were processed and analyzed by Biolab Laboratory (level V-SSN Excellence: ISO 9001:2015), Biolab Srl (Ascoli Piceno, Italy). DNA extraction, library preparation, and sequencing were performed in all samples. After sequencing, low-quality and polyclonal sequences were filtered out by the Ion software. At this point, we employed Kraken2 for microbial community analysis in keratitis samples. Nuclease-free water and all the reagents included in the experiment were used as a negative control. The primary outcome was the reduction in bacterial DNA (microbial load) at T1, expressed as a percentage of the baseline value (T0). Richness and Shannon alpha diversity metrics, along with Bray-Curtis beta diversity values, were calculated using the phyloseq package in R. Principal coordinate analysis was also conducted to interpret these metrics. Results : 19 samples were included in the study. The results exhibited a motley species richness, with the highest recorded value surpassing 800 species. Most of the samples displayed richness values ranging broadly from under 200 to around 600, indicating considerable variability in species count among the keratitis samples. Conclusions: A significant presence of both typical and atypical bacterial phyla in keratitis infections, underlining the complexity of the disease's microbial etiology. |
