Multiprotein collagen/keratin hydrogel promoted myogenesis and angiogenesis of injured skeletal muscles in a mouse model.

Autor: Namjoo AR; Stem Cell Research Center, Tabriz University of Medical Sciences, Imam Reza St, Golgasht St, Tabriz, Iran., Hassani A; Chemical Engineering Faculty, Sahand University of Technology, Tabriz, 51335-1996, Iran.; Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran., Amini H; Department of General and Vascular Surgery, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran., Nazaryabrbekoh F; Stem Cell Research Center, Tabriz University of Medical Sciences, Imam Reza St, Golgasht St, Tabriz, Iran., Saghati S; Department of Tissue Engineering, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran., Saadatlou MAE; Department of Basic Sciences, College of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran. ebrahimi@iaut.ac.ir., Khoshfetrat AB; Chemical Engineering Faculty, Sahand University of Technology, Tabriz, 51335-1996, Iran., Khosrowshahi ND; Chemical Engineering Faculty, Sahand University of Technology, Tabriz, 51335-1996, Iran., Rahbarghazi R; Stem Cell Research Center, Tabriz University of Medical Sciences, Imam Reza St, Golgasht St, Tabriz, Iran. rezarahbardvm@gmail.com.; Department of Applied Cell Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran. rezarahbardvm@gmail.com.
Jazyk: angličtina
Zdroj: BMC biotechnology [BMC Biotechnol] 2024 Apr 26; Vol. 24 (1), pp. 23. Date of Electronic Publication: 2024 Apr 26.
DOI: 10.1186/s12896-024-00847-4
Abstrakt: Volumetric loss is one of the challenging issues in muscle tissue structure that causes functio laesa. Tissue engineering of muscle tissue using suitable hydrogels is an alternative to restoring the physiological properties of the injured area. Here, myogenic properties of type I collagen (0.5%) and keratin (0.5%) were investigated in a mouse model of biceps femoris injury. Using FTIR, gelation time, and rheological analysis, the physicochemical properties of the collagen (Col)/Keratin scaffold were analyzed. Mouse C2C12 myoblast-laden Col/Keratin hydrogels were injected into the injury site and histological examination plus western blotting were performed to measure myogenic potential after 15 days. FTIR indicated an appropriate interaction between keratin and collagen. The blend of Col/Keratin delayed gelation time when compared to the collagen alone group. Rheological analysis revealed decreased stiffening in blended Col/Keratin hydrogel which is favorable for the extrudability of the hydrogel. Transplantation of C2C12 myoblast-laden Col/Keratin hydrogel to injured muscle tissues led to the formation of newly generated myofibers compared to cell-free hydrogel and collagen groups (p < 0.05). In the C2C12 myoblast-laden Col/Keratin group, a low number of CD31 + cells with minimum inflammatory cells was evident. Western blotting indicated the promotion of MyoD in mice that received cell-laden Col/Keratin hydrogel compared to the other groups (p < 0.05). Despite the increase of the myosin cell-laden Col/Keratin hydrogel group, no significant differences were obtained related to other groups (p > 0.05). The blend of Col/Keratin loaded with myoblasts provides a suitable myogenic platform for the alleviation of injured muscle tissue.
(© 2024. The Author(s).)
Databáze: MEDLINE
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