Reduced ossification caused by 3D simulated microgravity exposure is short-term in larval zebrafish.

Autor: Carvajal-Agudelo JD; Department of Biology, Mount Saint Vincent University, Halifax, NS, B3M 2J6, Canada., Eaton J; Department of Biology, Mount Saint Vincent University, Halifax, NS, B3M 2J6, Canada; Department of Biology, Saint Mary's University, Halifax, NS, Canada., Franz-Odendaal TA; Department of Biology, Mount Saint Vincent University, Halifax, NS, B3M 2J6, Canada. Electronic address: tamara.franz-odendaal@msvu.ca.
Jazyk: angličtina
Zdroj: Life sciences in space research [Life Sci Space Res (Amst)] 2024 May; Vol. 41, pp. 127-135. Date of Electronic Publication: 2024 Feb 23.
DOI: 10.1016/j.lssr.2024.02.006
Abstrakt: Understanding how skeletal tissues respond to microgravity is ever more important with the increased interest in human space travel. Here, we exposed larval Danio rerio at 3.5 dpf to simulated microgravity (SMG) using a 3D mode of rotation in a ground-based experiment and then studied different cellular, molecular, and morphological bone responses both immediately after exposure and one week later. Our results indicate an overall decrease in ossification in several developing skeletal elements immediately after SMG exposure with the exception of the otoliths, however ossification returns to normal levels seven days after exposure. Coincident with the reduction in overall ossification tnfsf11 (RANKL) expression is highly elevated after 24 h of SMG exposure and also returns to normal levels seven days after exposure. We also show that genes associated with osteoblasts are unaffected immediately after SMG exposure. Thus, the observed reduction in ossification is primarily the result of a high level of bone resorption. This study sheds insight into the nuances of how osteoblasts and osteoclasts in the skeleton of a vertebrate organism respond to an external environmental disturbance, in this case simulated microgravity.
Competing Interests: Declaration of competing interest The authors declare no competing interests.
(Copyright © 2024 The Committee on Space Research (COSPAR). Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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