Molecular characterization of Lernathropus kroyeri from intensive aquaculture and pathophysiology of infested sea bass.

Autor: Lattos A; Department of Zoology, School of Biology, Aristotle University of Thessaloniki, GR-54124, Thessaloniki, Greece., Makri V; Department of Zoology, School of Biology, Aristotle University of Thessaloniki, GR-54124, Thessaloniki, Greece., Papadopoulos DK; Department of Zoology, School of Biology, Aristotle University of Thessaloniki, GR-54124, Thessaloniki, Greece., Gourzioti E; Department of Aquaculture and Fish Diseases, School of Health Sciences, University of Thessaly, GR-43100, Karditsa, Greece., Pagonis C; Department of Aquaculture and Fish Diseases, School of Health Sciences, University of Thessaly, GR-43100, Karditsa, Greece., Georgoulis I; Department of Zoology, School of Biology, Aristotle University of Thessaloniki, GR-54124, Thessaloniki, Greece., Karagiannis D; Laboratory of Ichthyology - Culture and Pathology of Aquatic Animals, Aristotle University of Thessaloniki, GR-54124, Thessaloniki, Greece., Theodorou JA; Department of Fisheries and Aquaculture, School of Agricultural Sciences, University of Patras, GR-26504, Mesolonghi, Greece., Michaelidis B; Department of Zoology, School of Biology, Aristotle University of Thessaloniki, GR-54124, Thessaloniki, Greece., Giantsis IA; Department of Animal Science, Forestry and Natural Environment, Aristotle University of Thessaloniki, GR-54124, Thessaloniki, Greece., Feidantsis K; Department of Fisheries and Aquaculture, School of Agricultural Sciences, University of Patras, GR-26504, Mesolonghi, Greece. Electronic address: kfeidant@upatras.gr.
Jazyk: angličtina
Zdroj: Fish & shellfish immunology [Fish Shellfish Immunol] 2024 Jun; Vol. 149, pp. 109576. Date of Electronic Publication: 2024 Apr 25.
DOI: 10.1016/j.fsi.2024.109576
Abstrakt: The copepod Lernathropus kroyeri constitutes one of the major parasites for the Mediterranean aquaculture, infesting the sea bass Dicentrarchus labrax causing thus disruptions of growth performance and occasionally mortalities. Despite the large spread and the high frequency of this parasite in mariculture farms of Eastern Mediterranean, L. kroyeri genetic profile from aquaculture as well as the pathophysiological response of D. labrax have not been studied so far. Keeping this in mind, in the present study we investigated the L. kroyeri infestation on D. labrax from two farms in Greece, examining both healthy and heavy parasitized individuals. Assays included histopathology, phylogenetic reconstruction of the parasite and physiological response of the fish by the means of antioxidant, inflammatory metabolic and stress related gene expression analysis at both mRNA and protein levels. Genetic analysis indicated that L. kroyeri composes a monophyletic group, highly phylogenetically distant from other congeneric groups. Heavy infested D. labrax witnessed a significantly increased immune response that further led to oxidative stress and metabolic alterations. Overall, our results demonstrate the, seasonally independent, high infestation of this parasitic copepods, which continue to affect Mediterranean intensive aquaculture systems.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:John A. Theodorou reports financial support was provided by EU - Greece Operational Program. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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