Human serum albumin directed formation of cadmium telluride quantum dots: Applications in biosensing, anti-bacterial activities and cell cytotoxicity measurements.

Autor: Haque M; Department of Chemistry, National Institute of Technology Meghalaya, Shillong 793003, India., Kalita M; Department of Chemistry, National Institute of Technology Meghalaya, Shillong 793003, India., Chamlagai D; Department of Chemistry, North-Eastern Hill University, Shillong 793022, India., Lyndem S; Department of Chemistry, National Institute of Technology Meghalaya, Shillong 793003, India., Koley S; Department of Physics, Amity Institute of Applied Sciences, Amity University, Kolkata 700135, India., Kumari P; Department of Biotechnology & Bioinformatics, North-Eastern Hill University, Shillong 793022, India., Aguan K; Department of Biotechnology & Bioinformatics, North-Eastern Hill University, Shillong 793022, India., Singha Roy A; Department of Chemistry, National Institute of Technology Meghalaya, Shillong 793003, India. Electronic address: asroy86@nitm.ac.in.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 May; Vol. 268 (Pt 1), pp. 131862. Date of Electronic Publication: 2024 Apr 24.
DOI: 10.1016/j.ijbiomac.2024.131862
Abstrakt: Although cadmium-based quantum dots (QDs) are highly promising candidates for numerous biological applications, their intrinsic toxicity limits their pertinency in living systems. Surface functionalization of QDs with appropriate molecules could reduce the toxicity level. Herein, we have synthesized the smaller sized (1-5 nm) aqueous-compatible biogenic CdTe QDs using human serum albumin (HSA) as a surface passivating agent via a greener approach. HSA-functionalized CdTe QDs have been explored in multiple in vitro sensing and biological applications, namely, (1) sensing, (2) anti-bacterial and (3) anti-cancer properties. Using CdTe-HSA QDs as a fluorescence probe, a simple fluorometric method has been developed for highly sensitive and selective detection of blood marker bilirubin and hazardous Hg 2+ ion with a limit of detection (LOD) of 3.38 and 0.53 ng/mL, respectively. CdTe-HSA QDs also acts as a sensor for standard antibiotics, tetracycline and rifampicin with LOD values of 41.34 and 114.99 ng/mL, respectively. Nano-sized biogenic CdTe-HSA QDs have shown promising anti-bacterial activities against both gram-negative, E. coli and gram-positive, E. faecalis strains confirming more effectiveness against E. faecalis strains. The treatment of human cervical cancer cell lines (HeLa cells) with the synthesized QDs reflected the proficient cytotoxic properties of QDs.
Competing Interests: Declaration of competing interest The authors declare that no known competing conflict of financial interests or personal relationships exists, that could bias the reported work. All authors have read and approved this submission.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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