Recent advances in minimal fluorescent probes for optical imaging.

Autor: de Moliner F; Centre for Inflammation Research, The University of Edinburgh, EH16 4UU Edinburgh, UK; IRR Chemistry Hub, Institute for Regeneration and Repair, The University of Edinburgh, EH16 4UU Edinburgh, UK., Nadal-Bufi F; Centre for Inflammation Research, The University of Edinburgh, EH16 4UU Edinburgh, UK; IRR Chemistry Hub, Institute for Regeneration and Repair, The University of Edinburgh, EH16 4UU Edinburgh, UK., Vendrell M; Centre for Inflammation Research, The University of Edinburgh, EH16 4UU Edinburgh, UK; IRR Chemistry Hub, Institute for Regeneration and Repair, The University of Edinburgh, EH16 4UU Edinburgh, UK. Electronic address: marc.vendrell@ed.ac.uk.
Jazyk: angličtina
Zdroj: Current opinion in chemical biology [Curr Opin Chem Biol] 2024 Jun; Vol. 80, pp. 102458. Date of Electronic Publication: 2024 Apr 25.
DOI: 10.1016/j.cbpa.2024.102458
Abstrakt: Fluorescent probes have revolutionized biological imaging by enabling the real-time visualization of cellular processes under physiological conditions. However, their size and potential perturbative nature can pose challenges in retaining the integrity of biological functions. This manuscript highlights recent advancements in the development of small fluorescent probes for optical imaging studies. Single benzene-based fluorophores offer versatility with minimal disruption, exhibiting diverse properties like aggregation-induced emission and pH responsiveness. Fluorescent nucleobases enable precise labeling of nucleic acids without compromising function, offering high sensitivity and compatibility with biochemistry studies. Bright yet small fluorescent amino acids provide an interesting alternative to bulky fusion proteins, facilitating non-invasive imaging of cellular events with high precision. These miniaturized fluorophores promise enhanced capabilities for studying biological systems in a non-invasive manner, fostering further innovations in molecular imaging.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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