Cervical microbiota dysbiosis associated with high-risk Human Papillomavirus infection.

Autor: Zeber-Lubecka N; Department of Gastroenterology, Hepatology and Clinical Oncology, Centre of Postgraduate Medical Education, Warsaw, Poland.; Department of Genetics, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, Poland., Kulecka M; Department of Gastroenterology, Hepatology and Clinical Oncology, Centre of Postgraduate Medical Education, Warsaw, Poland.; Department of Genetics, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, Poland., Dabrowska M; Department of Genetics, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, Poland., Baginska-Drabiuk K; Department of Genetics, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, Poland., Glowienka-Stodolak M; Department of Genetics, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, Poland., Nowakowski A; Department of Cancer Prevention, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, Poland., Slabuszewska-Jozwiak A; First Department of Obstetrics and Gynecology, Centre of Postgraduate Medical Education, Warsaw, Poland., Bednorz B; Department of Cancer Prevention, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, Poland., Jędrzejewska I; Department of Cancer Prevention, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, Poland., Piasecka M; Department of Cancer Prevention, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, Poland., Pawelec J; Department of Cancer Prevention, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, Poland., Wojciechowska-Lampka E; Department of Lymphoid Malignancies, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, Poland., Ostrowski J; Department of Gastroenterology, Hepatology and Clinical Oncology, Centre of Postgraduate Medical Education, Warsaw, Poland.; Department of Genetics, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, Poland.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Apr 26; Vol. 19 (4), pp. e0302270. Date of Electronic Publication: 2024 Apr 26 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0302270
Abstrakt: High-risk Human Papillomavirus (HR-HPV) genotypes, specifically HPV16 and HPV18, pose a significant risk for the development of cervical intraepithelial neoplasia and cervical cancer. In the multifaceted cervical microenvironment, consisting of immune cells and diverse microbiota, Lactobacillus emerges as a pivotal factor, wielding significant influence in both stabilizing and disrupting the microbiome of the reproductive tract. To analyze the distinction between the cervical microbiota and Lactobacillus-dominant/non-dominant status of HR-HPV and non-infected healthy women, sixty-nine cervical swab samples were analyzed, included 44 with HR-HPV infection and healthy controls. All samples were recruited from Human Papillomavirus-based cervical cancer screening program and subjected to 16s rRNA sequencing analysis. Alpha and beta diversity analyses reveal no significant differences in the cervical microbiota of HR-HPV-infected women, including 16 and 18 HPV genotypes, and those with squamous intraepithelial lesion (SIL), compared to a control group. In this study we identified significantly lower abundance of Lactobacillus mucosae in women with HR-HPV infection compared to the control group. Furthermore, changes in bacterial diversity were noted in Lactobacillus non-dominant (LND) samples compared to Lactobacillus-dominant (LD) in both HR-HPV-infected and control groups. LND samples in HR-HPV-infected women exhibited a cervical dysbiotic state, characterized by Lactobacillus deficiency. In turn, the LD HR-HPV group showed an overrepresentation of Lactobacillus helveticus. In summary, our study highlighted the distinctive roles of L. mucosae and L. helveticus in HR-HPV infections, signaling a need for further research to demonstrate potential clinical implications of cervical microbiota dysbiosis.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Zeber-Lubecka et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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