Long-Term Culturing of Placozoans (Trichoplax and Hoilungia).
| Autor: | Romanova DY; Institute of Higher Nervous Activity and Neurophysiology of RAS, Moscow, Russia. darjaromanova@gmail.com., Varoqueaux F; Department of Fundamental Neurosciences, University of Lausanne, Lausanne, Switzerland. Frederique.Varoqueaux@unil.ch., Eitel M; Department of Earth and Environmental Sciences Palaeontology & Geobiology, LMU München, Munich, Germany., Yoshida MA; Marine Biological Science Section, Education and Research Center for Biological Resources, Faculty of Life and Environmental Science, Shimane University, Okinoshima, Oki, Shimane, Japan., Nikitin MA; Institute of Higher Nervous Activity and Neurophysiology of RAS, Moscow, Russia.; Belozersky Institute for Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.; Kharkevich Institute for Information Transmission Problems, RAS, Moscow, Russia., Moroz LL; Department of Neuroscience, McKnight Brain Institute, University of Florida, Gainesville, FL, USA. moroz@whitney.ufl.edu.; Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, FL, USA. moroz@whitney.ufl.edu. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2757, pp. 509-529. |
| DOI: | 10.1007/978-1-0716-3642-8_21 |
| Abstrakt: | The phylum Placozoa remains one of the least explored among early-branching metazoan lineages. For over 130 years, this phylum had been represented by the single species Trichoplax adhaerens-an animal with the simplest known body plan (three cell layers without any organs) but complex behaviors. Recently, extensive sampling of placozoans across the globe and their subsequent genetic analysis have revealed incredible biodiversity with numerous cryptic species worldwide. However, only a few culture protocols are available to date, and all are for one species only. Here, we describe the breeding of four different species representing two placozoan genera: Trichoplax adhaerens, Trichoplax sp. H2, Hoilungia sp. H4, and Hoilungia hongkongensis originating from diverse biotopes. Our protocols allow to culture all species under comparable conditions. Next, we outlined various food sources and optimized strain-specific parameters enabling long-term culturing. These protocols can facilitate comparative analyses of placozoan biology and behaviors, which together will contribute to deciphering general principles of animal organization. (© 2024. Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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