Stable Laboratory Culture System for the Ctenophore Mnemiopsis leidyi.
| Autor: | Soto-Angel JJ; Michael Sars Centre, University of Bergen, Bergen, Norway. joan.soto@uib.no., Nordmann EL; Michael Sars Centre, University of Bergen, Bergen, Norway., Sturm D; University of Plymouth, Plymouth, UK., Sachkova M; Michael Sars Centre, University of Bergen, Bergen, Norway., Pang K; Michael Sars Centre, University of Bergen, Bergen, Norway., Burkhardt P; Michael Sars Centre, University of Bergen, Bergen, Norway. pawel.burkhardt@uib.no. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2757, pp. 123-145. |
| DOI: | 10.1007/978-1-0716-3642-8_4 |
| Abstrakt: | Ctenophores are marine organisms attracting significant attention from evolutionary biology, molecular biology, and ecological research. Here, we describe an easy and affordable setup to maintain a stable culture of the ctenophore Mnemiopsis leidyi. The challenging delicacy of the lobate ctenophores can be met by monitoring the water quality, providing the right nutrition, and adapting the handling and tank set-up to their fragile gelatinous body plan. Following this protocol allows stable laboratory lines, a continuous supply of embryos for molecular biological studies, and independence from population responses to environmental fluctuations. (© 2024. Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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