Integrated Single Cell Analysis Reveals An Atlas of Tumor Associated Macrophages in Hepatocellular Carcinoma.

Autor: Li X; Organ Transplantation Center, Affiliated Hospital of Qingdao University, Qingdao, China.; Institute of Organ Donation and Transplantation, Medical College of Qingdao University, Qingdao, China., Li R; Department of Pulmonary and Critical Care Medicine, Second Affiliated Hospital of Harbin Medical University, Harbin, China., Miao X; Organ Transplantation Center, Affiliated Hospital of Qingdao University, Qingdao, China.; Institute of Organ Donation and Transplantation, Medical College of Qingdao University, Qingdao, China., Zhou X; Organ Transplantation Center, Affiliated Hospital of Qingdao University, Qingdao, China.; Institute of Organ Donation and Transplantation, Medical College of Qingdao University, Qingdao, China., Wu B; Organ Transplantation Center, Affiliated Hospital of Qingdao University, Qingdao, China.; Institute of Organ Donation and Transplantation, Medical College of Qingdao University, Qingdao, China., Cao J; Organ Transplantation Center, Affiliated Hospital of Qingdao University, Qingdao, China.; Institute of Organ Donation and Transplantation, Medical College of Qingdao University, Qingdao, China., Wang C; Organ Transplantation Center, Affiliated Hospital of Qingdao University, Qingdao, China.; Institute of Organ Donation and Transplantation, Medical College of Qingdao University, Qingdao, China., Li S; Department of Hepatopancreaticobiliary Surgery, Henan Provincial People's Hospital, Zhengzhou University, Zhengzhou, China. shipengli2010@163.com., Cai J; Organ Transplantation Center, Affiliated Hospital of Qingdao University, Qingdao, China. caijinzhen@qdu.edu.cn.; Institute of Organ Donation and Transplantation, Medical College of Qingdao University, Qingdao, China. caijinzhen@qdu.edu.cn.
Jazyk: angličtina
Zdroj: Inflammation [Inflammation] 2024 Dec; Vol. 47 (6), pp. 2077-2093. Date of Electronic Publication: 2024 Apr 26.
DOI: 10.1007/s10753-024-02026-1
Abstrakt: Hepatocellular carcinoma (HCC), one of the most prevalent cancers globally, is closely associated with tumor-associated macrophages (TAMs), including monocyte-derived macrophages and liver-resident Kupffer cells. Understanding TAM heterogeneity at the cellular level is crucial for developing effective HCC prevention and treatment strategies. In this study, we conducted an integrated single-cell analysis of four cohorts (GSE140228, GSE125449, GSE149614 and GSE156625) to elucidate the TAM landscape in HCC. We identified 284 gene markers, termed Panmyeloid markers, that characterize myeloid cells within this context. Our analysis distinguished six clusters of monocyte-derived macrophages (Macro1-Macro6) and four clusters of Kupffer cells (Kupffer1-Kupffer4). Notably, CXCL10 + macrophages and MT1G + Kupffer cells, predominantly located within tumor tissues, exhibited distinct functional characteristics relevant to HCC. We also explored cellular communication between TAMs and T cells, uncovering potential signaling pathways such as the CXCL10/CXCL11-CXCR3 and CXCL12-CXCR4 networks. These findings enhance our understanding of TAMs in HCC and open new avenues for targeted therapeutic interventions.
Competing Interests: Declarations. Ethics approval and consent to participate: The study protocol was approved by the ethics committee of Affiliated Hospital of Qingdao University. All subjects provided informed consent and all experiments were conducted per the study protocol. Consent for publication: All authors have reviewed the manuscript and have given consent for publication. Competing interests: The authors declare no conflicts of interest in this work.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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