Autor: |
Grasso F; Department of Pharmacy, University of Genova, Viale Cembrano 4, 16148 Genova, Italy., Méndez Paz D; ANFACO-CECOPESCA, Department of Circular Economy, Colexio Universitario, 36310 Vigo, Spain., Vázquez Sobrado R; ANFACO-CECOPESCA, Department of Circular Economy, Colexio Universitario, 36310 Vigo, Spain., Orlandi V; Department of Pharmacy, University of Genova, Viale Cembrano 4, 16148 Genova, Italy., Turrini F; Department of Pharmacy, University of Genova, Viale Cembrano 4, 16148 Genova, Italy.; National Center for the Development of New Technologies in Agriculture (Agritech), 80121 Napoli, Italy., Agostinis L; Aimplas, Asociación de Investigación de Materiales Plásticos Y Conexas, Carrer de Gustave Eiffel, 4, 46980 Valencia, Spain., Morandini A; Aimplas, Asociación de Investigación de Materiales Plásticos Y Conexas, Carrer de Gustave Eiffel, 4, 46980 Valencia, Spain., Jenssen M; Nofima, Muninbakken, 9-13, 9019 Tromsø, Norway., Lian K; Nofima, Muninbakken, 9-13, 9019 Tromsø, Norway., Boggia R; Department of Pharmacy, University of Genova, Viale Cembrano 4, 16148 Genova, Italy.; National Biodiversity Future Center (NBFC), 90133 Palermo, Italy. |
Abstrakt: |
The enzymatic extraction of proteins from fish biomasses is being widely investigated. However, little or almost no research has paid attention to the exploitation of unsorted fishery biomasses. This work is part of a larger study, Part I of which has already been published, and focuses on an extensive characterization of two collagenous samples, namely gelatin (G) and hydrolyzed gelatin peptides (HGPs), extracted from a dehydrated fish biomass coming from unsorted canned yellowfin tuna side streams. The results indicate crude protein fractions of 90-93%, pH values between 3 and 5, white-yellow colors, collagen-like FTIR spectra, and 17% in terms of total amino acid content. Viscosity and the study of dynamic viscous-elastic behavior were analyzed. Thermo-gravimetric analysis was performed to assess the residual ashes. Both samples were investigated to determine their molecular weight distribution via size-exclusion chromatography, with a higher total average molecular weight for G compared to HGPs, with values of 17,265.5 Da and 2637.5 Da, respectively. G demonstrated technological properties similar to analogous marine gelatins. HGPs demonstrated antioxidant activity as per FRAP assay. All the results open up new perspectives for the potential use of these substances in biodegradable packaging, dietary supplements, and skin care cosmetics. |