| Autor: |
Tóth K; Institute of Medical Microbiology, Faculty of Medicine, Semmelweis University, 1089 Budapest, Hungary.; Department of Bacteriology, Parasitology and Mycology, National Center for Public Health and Pharmacy, 1097 Budapest, Hungary., Damjanova I; Department of Bacteriology, Parasitology and Mycology, National Center for Public Health and Pharmacy, 1097 Budapest, Hungary., Laczkó L; One Health Institute, Faculty of Health Sciences, University of Debrecen, 4032 Debrecen, Hungary.; HUN-REN-DE Conservation Biology Research Group, University of Debrecen, 4032 Debrecen, Hungary., Buzgó L; Department of Bacteriology, Parasitology and Mycology, National Center for Public Health and Pharmacy, 1097 Budapest, Hungary., Lesinszki V; Department of Bacteriology, Parasitology and Mycology, National Center for Public Health and Pharmacy, 1097 Budapest, Hungary., Ungvári E; Department of Bacteriology, Parasitology and Mycology, National Center for Public Health and Pharmacy, 1097 Budapest, Hungary., Jánvári L; Department of Bacteriology, Parasitology and Mycology, National Center for Public Health and Pharmacy, 1097 Budapest, Hungary., Hanczvikkel A; Department of Bacteriology, Parasitology and Mycology, National Center for Public Health and Pharmacy, 1097 Budapest, Hungary., Tóth Á; Department of Bacteriology, Parasitology and Mycology, National Center for Public Health and Pharmacy, 1097 Budapest, Hungary., Szabó D; Institute of Medical Microbiology, Faculty of Medicine, Semmelweis University, 1089 Budapest, Hungary.; HUN-REN-SE Human Microbiota Research Group, 1052 Budapest, Hungary.; Neurosurgical and Neurointervention Clinic, Semmelweis University, 1083 Budapest, Hungary. |
| Abstrakt: |
Extended-spectrum β-lactamase-producing Escherichia coli ST131 has become widespread worldwide. This study aims to characterize the virulome, resistome, and population structure of E. coli ST131 isolates from clinical blood samples in Hungary. A total of 30 C2/H30Rx and 33 C1-M27 ST131 isolates were selected for Illumina MiSeq sequencing and 30 isolates for MinION sequencing, followed by hybrid de novo assembly. Five C2/H30Rx and one C1-M27 cluster were identified. C1-M27 isolates harbored the F1:A2:B20 plasmid in 93.9% of cases. Long-read sequencing revealed that bla CTX-M-27 was on plasmids. Among the C2/H30Rx isolates, only six isolates carried the C2-associated F2:A1:B- plasmid type. Of 19 hybrid-assembled C2/H30Rx genomes, the bla CTX-M-15 gene was located on plasmid only in one isolate, while in the other isolates, IS Ecp 1 or IS 26 -mediated chromosomal integration of bla CTX-M-15 was detected in unique variations. In one isolate a part of F2:A1:B- plasmid integrated into the chromosome. These results suggest that CTX-M-15-producing C2/H30Rx and CTX-M-27-producing C1-M27 subclades may have emerged and spread in different ways in Hungary. While bla CTX-M-27 was carried mainly on the C1/H30R-associated F1:A2:B20 plasmid, the IncF-like plasmids of C2/H30Rx or its composite transposons have been incorporated into the chromosome through convergent evolutionary processes. |
