Long-term efficacy of liposomal nanocarriers of preassembled glycocalyx in restoring cerebral endothelial glycocalyx in sepsis.

Autor: Ishiko S; Department of Medicine, New York Medical College, 15 Dana Road, Valhalla, New York 10595, USA., Huang A; Department of Physiology, New York Medical College, 15 Dana Road, Valhalla, New York 10595, USA., Sun D; Department of Physiology, New York Medical College, 15 Dana Road, Valhalla, New York 10595, USA. Electronic address: dong_sun@nymc.edu.
Jazyk: angličtina
Zdroj: Microvascular research [Microvasc Res] 2024 Jul; Vol. 154, pp. 104684. Date of Electronic Publication: 2024 Apr 23.
DOI: 10.1016/j.mvr.2024.104684
Abstrakt: The endothelial glycocalyx (EG) undergoes early degradation in sepsis. Our recent work introduced a novel therapeutic approach involving liposomal nanocarriers of preassembled glycocalyx (LNPG) to restore EG in lipopolysaccharide (LPS)-induced sepsis model of mice. While short-term effects were promising, this study focuses on the long-term impact of LNPG on mouse cerebral microcirculation. Utilizing cranial window, we assessed the stability of vascular density (VD) and perfused boundary region (PBR), an index of EG thickness, over a five-day period in normal control mice. In septic groups (LPS, LPS + 1-dose LNPG, and LPS + 2-dose LNPG), the exposure of mice to LPS significantly reduced VD and increased PBR within 3 h. Without LNPG treatment, PBR returned to the normal control level by endogenous processes at 48 h, associated with the recovery of VD to the baseline level at 72 h. However, mice receiving LNPG treatment significantly reduced the increment of PBR at 3 h. The therapeutic effect of 1-dose LNPG persisted for 6 h while the 2-dose LNPG treatment further reduced PBR and significantly increased VD at 12 h compared to LPS group. This study provides valuable insights into the potential therapeutic benefits of LNPG in mitigating EG degradation in sepsis.
Competing Interests: Declaration of competing interest The Authors declare no conflict of interest.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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