Long-term glucocorticoid infusion impairs epididymal adipocyte metabolism and maturation and affects miR-150-5p actions.

Autor: Salgueiro RB; University of São Paulo, Institute of Biomedical Sciences, Department of Physiology and Biophysics, São Paulo, SP, Brazil; University of Brasília, Biology Institute, Department of Physiological Sciences, Brasília, Federal District, Brazil. Electronic address: rafaeleefe@yahoo.com.br., Bolin AP; University of São Paulo, Institute of Biomedical Sciences, Department of Pharmacology, São Paulo, SP, Brazil., Andreotti S; University of São Paulo, Institute of Biomedical Sciences, Department of Physiology and Biophysics, São Paulo, SP, Brazil., Medeiros Komino AC; University of São Paulo, Institute of Biomedical Sciences, Department of Physiology and Biophysics, São Paulo, SP, Brazil., de Sousa É; University of São Paulo, Institute of Biomedical Sciences, Department of Pharmacology, São Paulo, SP, Brazil., de Fatima Silva F; University of São Paulo, Institute of Biomedical Sciences, Department of Physiology and Biophysics, São Paulo, SP, Brazil., Gomes de Proença AR; Faculdades Integradas Einstein de Limeira, São Paulo, SP, Brazil., Laurato Sertié RA; University of São Paulo, Institute of Biomedical Sciences, Department of Physiology and Biophysics, São Paulo, SP, Brazil., Rodrigues AC; University of São Paulo, Institute of Biomedical Sciences, Department of Pharmacology, São Paulo, SP, Brazil., Lima FB; University of São Paulo, Institute of Biomedical Sciences, Department of Physiology and Biophysics, São Paulo, SP, Brazil.
Jazyk: angličtina
Zdroj: Molecular and cellular endocrinology [Mol Cell Endocrinol] 2024 Aug 01; Vol. 589, pp. 112250. Date of Electronic Publication: 2024 Apr 23.
DOI: 10.1016/j.mce.2024.112250
Abstrakt: The most common form of hypercortisolism is iatrogenic Cushing's syndrome. Lipodystrophy and metabolic disorders can result from the use of exogenous glucocorticoids (GC). Adipocytes play an important role in the production of circulating exosomal microRNAs, and knockdown of Dicer promotes lipodystrophy. The aim of this study is to investigate the effect of GCs on epididymal fat and to assess their influence on circulating microRNAs associated with fat turnover. The data indicate that despite the reduction in adipocyte volume due to increased lipolysis and apoptosis, there is no difference in tissue mass, suggesting that epididymal fat pad, related to animal size, is not affected by GC treatment. Although high concentrations of GC have no direct effect on epididymal microRNA-150-5p expression, GC can induce epididymal adipocyte uptake of microRNA-150-5p, which regulates transcription factor Ppar gamma during adipocyte maturation. In addition, GC treatment increased lipolysis and decreased glucose-derived lipid and glycerol incorporation. In conclusion, the similar control and GC epididymal fat mass results from increased dense fibrogenic tissue and decreased adipocyte volume induced by the lipolytic effect of GC. These findings demonstrate the complexity of epididymal fat. They also highlight how this disease alters fat distribution. This study is the first in a series published by our laboratory showing the detailed mechanism of adipocyte turnover in this disease.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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