Protocol for enrichment and functional analysis of human hematopoietic cells from umbilical cord blood.
| Autor: | Gutch S; Indiana University School of Medicine, Indianapolis, IN 46202, USA., Beasley L; Indiana University School of Medicine, Indianapolis, IN 46202, USA., Cooper S; Indiana University School of Medicine, Indianapolis, IN 46202, USA., Kaplan MH; Indiana University School of Medicine, Indianapolis, IN 46202, USA., Capitano ML; Indiana University School of Medicine, Indianapolis, IN 46202, USA., Ropa J; Indiana University School of Medicine, Indianapolis, IN 46202, USA. Electronic address: jropa@iu.edu. |
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| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2024 Jun 21; Vol. 5 (2), pp. 103024. Date of Electronic Publication: 2024 Apr 23. |
| DOI: | 10.1016/j.xpro.2024.103024 |
| Abstrakt: | Umbilical cord blood (CB) is a donor source for hematopoietic cell therapies. Understanding what drives hematopoietic stem and progenitor cell function is critical to our understanding of the usage of CB in hematopoietic cell therapies. Here, we describe how to isolate and analyze the function of human hematopoietic cells from umbilical CB. This protocol demonstrates assays that measure phenotypic properties and hematopoietic cell potency. For complete details on the use and execution of this protocol, please refer to Broxmeyer et al. 1 . Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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